BACKGROUND There is a general requirement to determine and correlate water content to viability for the standardization of conservation protocols to facilitate effective cryostorage of plant germplasm. OBJECTIVE This study examined water content as a critical factor to optimize the cryostorage of Allium sativum. MATERIALS AND METHODS Stem discs were excised from post-harvest, stored bulbs p...

In the present studies, an attempt has been made to develop a method of liquid nitrogen preservation of plant explants propagated in vitro in the laboratory of the Department of Ornamental Plants, of Agricultural University in Kraków: shoot apical and axillary meristems of Rosa ‘New Dawn’, somatic embryos of snowdrops Galanthus nivalis L. and G. elwesii Hook, and gametophyte of Phlebodium aureu...

Dry flower industry in floriculture is gaining knowledge with the booming India. Despite poor weather circumstances, dry sector gives an excellent chance for farmers to improve their income throughout year. Dehydration techniques help maintain original colour and shape of flowers long-term use little care. In addition that drying different plant parts epoxy resin encapsulation technique a novel...

In the current study, V6, V7 and V8 amyloses were prepared, their crystalline polymorphic transitions upon hydration at different moisture conditions examined by wide-angle X-ray diffraction (XRD), relative degrees of crystallinity calculated. Upon formation or crystals, XRD peaks shifted to lower Bragg angles as compared V6h due expansion amylose helices. Conditioning 75% equilibrium humidity ...

Shoot tips of cassava (Manihot esculenta Crantz) in vitro plantlets were successfully cryopreserved using the encapsulation-vitrification technique. Nodal cuttings of 5 mm length with one leaf were cultured on modified MS medium in Petri dishes (90 mm x 20 mm) for about 28 days. Excised shoot tips were precultured on sucrose enriched (0.3 M) medium for 16 h, encapsulated and osmoprotected with ...

The antimonial drug, meglumine antimoniate, was successfully encapsulated in dehydration-rehydration vesicles and in freeze-dried empty liposomes (FDELs). High encapsulation efficiencies (from 28 to 58%) and low weight ratios of lipids to encapsulated antimony (from 1:0.15 to 1:0.3) were achieved. These formulations, contrary to those obtained by conventional methods, can be stored as intermedi...