In this article the tools, techniques, and instruments appropriate for quantitative measurements of intracellular elemental content using the technique known as electron probe microanalysis (EPMA) are described. Intramitochondrial calcium is a particular focus because of the critical role that mitochondrial calcium overload plays in neurodegenerative diseases. The method is based on the analysi...

Atomic layer deposition (ALD) of aluminum oxide thin films were applied on lateral high-aspect-ratio silicon test structures. The leading front the film thickness profile is interest, since it related to kinetics and provides information for ALD process development. A deposited was characterized using energy-dispersive electron probe X-ray microanalysis (ED-EPMA) results analyzed Monte Carlo si...

Over the last decades, determination of ages in monazite using Electron Probe Microanalysis (EPMA) has progressed to the point that it is now considered as a routine procedure within several laboratories worldwide [1,2]. Nevertheless, the typical age errors of individual electron probe spots reported in the literature are an order of magnitude larger than attainable by Secondary Ion Mass Spectr...

Our previous histopathological study showed that the augmentation block, prepared from a calcium phosphate cement (CPC) mixed with H2O at powder to liquid ratio of 5 g/mL, placed on the alveolar bone ridge, was gradually replaced by natural bone. In the present study, fluorescent labeling analysis (FLA) and electron probe microanalysis (EPMA) were performed on the same surgical site of the abov...

Abstract Titanium alloys are widely used in the aerospace industry, yet oxygen ingress can severely degrade mechanical properties of titanium alloy components. Atom probe tomography (APT), electron microanalysis (EPMA) and nanoindentation were to characterise oxygen-rich layer on an in-service jet engine compressor disc, manufactured from TIMETAL 834. Oxygen was quantified related changes throu...

The accuracy of electron-probe microanalysis (EPMA) by wavelength-dispersive spectrometry (WDS) depends critically on spectrometer alignment, specimen positioning, pulse-height (PHA) discrimination, and deadtime correction, but also on the accurate measurement of characteristic x-ray intensities (i.e., “peak”) and continuum (i.e., “background”) which form the k-ratio that is converted by correc...

The generation of x rays from solid samples under electron bombardment is the basis of electron probe microanalysis (EPMA), a widely used technique for materials analysis. The problem of quantitative analysis, i.e. the transformation of the measured x-ray intensities into element concentrations, is generally solved by means of approximate, semi-empirical algorithms. These algorithms provide rel...

A cyclic wet/dry accelerated corrosion test was used to compare the corrosion behavior of medium-Mn steel and Q345 steel. In terms of scanning electron microscope (SEM), using X-ray diffraction (XRD), electron probe microanalysis (EPMA), X-ray photoelectron spectroscopy (XPS), and analysis of the corrosion process, the results showed that the medium-Mn steel did not exhibit higher corrosion res...