The polyamines putrescine, spermidine and spermine were analyzed in chick embryos during the first 2 days of development. A rapid increase in the activity of ornithine decarboxylase (ODC), the initial and rate-limiting enzyme in polyamine synthesis, was observed immediately after onset of incubation. Peak activities were found at 15 and 23 h of incubation. The first peak coincides with gastrula...

Polyamines are the natural compound, act as anti-senescent agents, reduce rate of respiration, delay ethylene production, retard colour change, maintain fruit firmness. Show that plays a pivotal role in postharvest life fruits and vegetables. The objective this study was to investigate effect post-harvest application polyamines different concentration on physico-physiological quality parameters...

Polyamines are polycationic molecules essential for cell growth and differentiation. Recent work has focused on cell polyamine-transport systems as a way to regulate intracellular polyamine levels. In this study, we demonstrate the presence of two different active transporters for putrescine and spermidine in a rat tumoral cell line (AR4-2J). The first has a Km of 3.1 microM and a Vmax of 3.7 p...

Polyamines are highly regulated polycations which are essentially involved in cell growth and differentiation. Polyamines in food significantly contribute to the polyamine body pool. Dietary polyamines exert various direct and indirect trophic effects on the rat's immature intestine and play an important role during intestinal maturation. Human milk and that of other mammalians contain relative...

The polyamines spermine and spermidine and the diamine putrescine inhibit lipid peroxidation in phospholipid liposome suspensions and rat liver homogenates. Using the chemiluminescence technique the antioxidant activity of polyamines was found to be due to reactions with the free radical intermediates of lipid peroxidation and/or superoxide radicals. Also, the antioxidant action of polyamines c...

An improved method of assay of urinary polyamines (putrescine, spermidine, and spermine) was applied to the study of cancer patients and an experimental gastric tumor of rats. Although total polyamines (putrescine, spermidine, and spermine) in urine of patients with blood and solid cancers were significantly high, putrescine concentrations also increased significantly and were shown to be of di...

The levels of free, perchloric acid-soluble conjugated and perchloric acid-insoluble bound polyamines were determined in pericarp and seeds of `Muscat Bailey A' grapes (Vitis labrusca L. Vitis vinifera L.) during development and ripening. In both, the pericarp and seeds, putrescine was the predominant polyamine in the three fractions, and the bound polyamine level was the highest of the fractio...

Ornithine decarboxylase (ODC) is feedback regulated by polyamines. ODC antizyme mediates this process by forming a complex with ODC and enhancing its degradation. It has been reported that polyamines induce ODC antizyme and inhibit ODC activity. Since exogenous polyamines can be converted to each other after they are taken up into cells, we used an inhibitor of S-adenosylmethionine decarboxylas...

Biogenic polyamines, such as putrescine, spermidine, and spermine are small organic polycations involved in numerous diverse biological processes. These compounds play an important role in nucleic acid function due to their binding to DNA and RNA. It has been shown that biogenic polyamines cause DNA condensation and aggregation similar to that of inorganic cobalt(III)hexamine cation, which has ...

This simple, rapid liquid-chromatographic assay of urinary polyamines (putrescine, spermidine, spermine, and cadaverine) involves electrochemical detection with a post-column immobilized enzyme, polyamine oxidase (EC 1.4.3.6) from soybean seedlings. Polyamines are separated by isocratic ion-pairing reversed-phase chromatography, then enzymatically converted, with release of hydrogen peroxide, v...