A simple spectrophotometric flow injection (FI) procedure for the determination of Cr(III) using ethylenediaminetetraacetic acid (EDTA) was developed. An FI system with a column packed with Amberlite IR-120(H) was employed for sample pretreatment. This leads to the possibility of a single standard calibration. A linear calibration in a range of 10-27 microg Cr(III) was obtained with a detection...

The pre-analytical, analytical and biological sources of variation were estimated for serum lipoprotein(a) concentrations, as measured with a new commercially available procedure. There were no significant differences between the concentrations of lipoprotein(a) measured in EDTA plasma and serum. The analyte is stable in specimens stored for up to two weeks in the refrigerator, or up to three m...

A novel sandwich ELISA for the quantitative and sensitive determination of IL-13 in human serum and plasma was established. The assay employs an incubation step at acidic pH, which was shown to decrease nonspecific binding and interference from IL-13 binding proteins. The assay was validated and was shown to be accurate and precise over the entire quantification range (0.59 to 68.4 pg/mL in hum...

A method has been developed for the rapid preparation of single-cell suspensions from rat hepatocyte primary cultures on collagen substratum. Hepatocytes were adequately dissociated into single cells when the cultures were first treated with a combination of trypsin and ethylenediaminetetraacetic acid (EDTA) and then with collagenase. However, when the order was reversed, hepatocytes were inade...

Fospropofol disodium (Lusedra) for anesthesia sedation.

OBJECTIVES This study was carried-out to evaluate and compare the efficacy of various irrigants when used singly or in combination on the microhardness of root canal dentin. STUDY DESIGN A total of 50 root-halves were randomly divided into 5 groups immediately after the initial baseline microhardness measurements and treated with: Group-1; 7.5%Ethylenediaminetetraaceticacid (EDTA) + 2.5%sodiu...

The main scope of this paper is first of all to verify the efficiency of the flame atomic absorption spectroscopy (FAAS) method for analysis of some trace elements in extracts of fractionation analysis of the gravitation dust sediments. Investigation was oriented to the analysis of some markedly toxic trace elements in the extracts of gravitation dust sediments as well as on efficiency evaluati...

Despite its environmental (and financial) importance, there is no agreement in the literature as to which extractant most accurately estimates the phytoavailability of trace metals in soils. A large data set was taken from the literature, and the effectiveness of various extractants to predict the phytoavailability of Cd, Zn, Ni, Cu, and Pb examined across a range of soil types and contaminatio...

The data reported indicate that the oxidation-reduction balance of the ascorbic acid system is not causally related to the auxin-regulation of cell elongation. There was no shift in the ascorbic acid (AA) to dehydroascorbic acid (DHA) ratio with growth-promoting concentration of auxin in several plant tissues. The AA to DHA ratio was experimentally increased without altering the growth rate. In...