As a response to high osmolality, many microorganisms synthesize various types of compatible solutes. These organic osmolytes aid in offsetting the detrimental effects of low water activity on cell physiology. One of these compatible solutes is ectoine. A sub-group of the ectoine producer's enzymatically convert this tetrahydropyrimidine into a hydroxylated derivative, 5-hydroxyectoine. This co...

Anaerobic digestion (AD) is a robust biotechnology for the valorisation of organic waste into biogas. However, rapid decrease in renewable electricity prices requires alternative uses In this context, engineering innovative platforms bio-production chemicals from CH4 has recently emerged. The extremolyte and osmoprotectant ectoine, with market price ~1000€/Kg, industrial flagship CH4-based bio-...

Gram-positive soil bacterium Corynebacterium glutamicum uses the compatible solutes glycine betaine, proline, and ectoine for protection against hyperosmotic shock. Osmoregulated glycine betaine carrier BetP and proline permease PutP have been previously characterized; we have identified and characterized two additional osmoregulated secondary transporters for compatible solutes in C. glutamicu...

Protein misfolding is considered a key event in the pathogenesis of polyglutamine disease such as Machado-Joseph disease (MJD). Overexpression of chaperone proteins and the application of chemical chaperones are reported to suppress polyglutamine induced cytotoxicity in vitro and in vivo. The effects of compatible solutes, which are osmoprotectants in bacteria and possess the action in stabiliz...

The cellular adjustment of Bacteria and Archaea to high-salinity habitats is well studied and has generally been classified into one of two strategies. These are to accumulate high levels either of ions (the "salt-in" strategy) or of physiologically compliant organic osmolytes, the compatible solutes (the "salt-out" strategy). Halophilic protists are ecophysiological important inhabitants of sa...

BACKGROUND Compatible solutes are natural substances that are known to stabilize cellular functions. Preliminary ex vivo and in vivo studies demonstrated that the compatible solute ectoine restores natural apoptosis rates of lung neutrophils and contributes to the resolution of lung inflammation. Due to the low toxicity and known compatibility of the substance, an inhalative application as an i...

Ectoine is a high-value stabilizer and protective agent with various applications in enzyme industry, cosmetics, biomedicine. In this study, rational engineering strategies have been implemented Escherichia coli to efficiently produce ectoine. First, the synthetic pathway of ectoine was constructed E. MG1655 by introducing an artificial thermal switch system harboring ectABC cluster from Halomo...

BACKGROUND Ectoine and its derivative 5-hydroxyectoine are cytoprotectants widely synthesized by microorganisms as a defense against the detrimental effects of high osmolarity on cellular physiology and growth. Both ectoines possess the ability to preserve the functionality of proteins, macromolecular complexes, and even entire cells, attributes that led to their description as chemical chapero...

Recycling of greenhouse gases to produce industrially valuable products has become one the big pillars achieve circularity. This study demonstrates for first time feasibility producing ectoine with Halomonas stevensii CO 2 as added carbon source, and glucose . Initially, alone was fed continuous reactors, adding thiosulphate energy source. Maximum elimination capacities 24.2 mg L ?1 h were obta...

Substrate-binding proteins or extracellular solute receptors (ESRs) are components of both ABC (ATP binding cassette) and TRAP-T (tripartite ATP-independent periplasmic transporter). The TRAP-T system UehABC from Silicibacter pomeroyi DSS-3 imports the compatible solutes ectoine and 5-hydroxyectoine as nutrients. UehA, the ESR of the UehABC operon, binds both ectoine and 5-hydroxyectoine with h...