Porphyrin photosensitizers tend to localize in mitochondria. The depolarization of mitochondrial membrane is one of the early stages of apoptosis and Laser Scanning Fluorescence Microscopy allows to determine changes in transmembrane mitochondrial potential under influence of PDT depending on the kind of photosensitizer (PP(Arg)(2), Hp(Arg)(2)), the energy dose (5, 10, 30 and 50 J/cm(2)) and ti...

Intracellular recordings were made from hippocampal CA1 neurons in rat slice preparations. Superfusion with oxygen- and glucose-deprived medium (in vitro ischemia) produced a rapid depolarization approximately 5 min after the onset of the superfusion. Even when oxygen and glucose were reintroduced immediately after rapid depolarization, the membrane depolarized further (persistent depolarizatio...

The relationships between damage-induced electropotential waves (EPWs), sieve tube occlusion, and stop of mass flow were investigated in intact Cucurbita maxima plants. After burning leaf tips, EPWs propagating along the phloem of the main vein were recorded by extra- and intracellular microelectrodes. The respective EPW profiles (a steep hyperpolarization/depolarization peak followed by a prol...

Spreading depolarization of cells in cerebral grey matter is characterized by massive ion translocation, neuronal swelling and large changes in direct current-coupled voltage recording. The near-complete sustained depolarization above the inactivation threshold for action potential generating channels initiates spreading depression of brain activity. In contrast, epileptic seizures show modest ...

The signaling mechanisms by which skeletal muscle electrical activity leads to changes in gene expression remain largely undefined. We have reported that myotube depolarization induces calcium signals in the cytosol and nucleus via inositol 1,4,5-trisphosphate (IP(3)) and phosphorylation of both ERK1/2 and cAMP-response element-binding protein (CREB). We now describe the calcium dependence of P...

The signaling mechanisms by which skeletal muscle electrical activity leads to changes in gene expression remain largely undefined. We have reported that myotubes depolarization induces calcium signals in cytosol and nucleus via inositol 1,4,5-trisphosphate (IP3) and phosphorylation of both ERKs 1/2 and cAMP-response element-binding protein (CREB). We now describe the calcium dependence of P-CR...

Li, Gui-Rong, Haiying Sun, and Stanley Nattel. Characterization of a transient outward K1 current with inward rectification in canine ventricular myocytes. Am. J. Physiol. 274 (Cell Physiol. 43): C577–C585, 1998.—The threshold potential for the classical depolarization-activated transient outward K1 current and Cl2 current is positive to 230 mV. With the whole cell patch technique, a transient ...

how to cite this article: kazemi h, speckmann ej, gorji a. familial hemiplegic migraine and spreading depression. iran j child neurol. 2014 summer;8(3): 6-11.   abstract objective familial hemiplegic migraine (fhm) is an autosomal dominantly inherited subtype of migraine with aura, characterized by transient neurological signs and symptoms. typical hemiplegic migraine attacks start in the first...

introduction: spreading depression (sd) is a propagating wave of depolarization followed by depression of the neuroglial activities and can modulate extracellular dopamine concentrations in the neocortex. it has been shown that the dopaminergic system plays a role in migraine. sd has been suggested as a critical phenomenon in the pathophysiology of migraine. the aim of this study was to investi...