The present study is concerned with the improvement of Bacillus licheniformis strain GCB30 for alpha amylase production in 250 ml Erlenmeyer flasks. Chemical mutation using ethyl methanesulfonate (EMS 50-300 μ/ml) was undertaken for 10-60 min. Twenty eight isolates were selected on the basis of clear zones of starch hydrolysis. Only one isolate designated as B. licheniformis EMS-200 gave 102.78...

Due to the limited availability of effective pharmaceutical products and serious side effects of the available therapy for leishmaniasis, search for useful medicinal plants is necessary. Previous studies reported preventative effect of Echinacea against Leishmania infections through immunologic stimulation. Here we tested concentrated ethanolic extract of the root of Echinacea purpurea prepared...

Inhibition of the proliferation of Ehrlichia risticii cultured in murine macrophage P388D1 cells by eight antibiotics was evaluated by indirect fluorescent-antibody staining with an antiserum specific to E. risticii. There was a negative correlation between the percentage of infected cells and the log10 of the concentrations of all antibiotics examined. The ranks of the antibiotics in the order...

Systemic anthrax manifests as toxemia, rapidly disseminating septicemia, immune collapse, and death. Virulence factors include the anti-phagocytic gamma-linked poly-d-glutamic acid (PGA) capsule and two binary toxins, complexes of protective antigen (PA) with lethal factor (LF) and edema factor. We report the characterization of LF, PA, and PGA levels during the course of inhalation anthrax in ...

BACKGROUND In hemodialysis, extracorporeal blood flow (Qb) recommendation is 300-500 mL/min. To achieve the best Qb, we based our prescription on dynamic arterial line pressure (DALP). METHODS This prospective study included 72 patients with catheter Group 1 (G1), 1877 treatments and 35 arterio-venous (AV) fistulae Group 2 (G2), 1868 treatments. The dialysis staff was trained to prescribe Qb ...

BACKGROUND Prostate cancer remains the most common malignancy among elderly men and the second leading cause of cancer death in the United States. Although several conventional therapies are currently available, they have a low efficacy and the more effective treatment modalities need to be established. Interferons (IFNs) are one of such options known as immunotherapy and demonstrated their ant...

Cocoa Ethanolic Extract (CEE) was observed for antibacterial activity against Escherichia coli. CEE at different concentrations in E. coli-seeded Mueller-Hinton agar medium, resulted zone of inhibition after 24 h incubation in 37°C. CEE concentrations at 15.6, 31.25, 62.5, 125, 250, 500 and 1,000 mg/ml, developed annular radius of inhibition 0.4, 1.0, 1.9, 3.3, 4.5, 5.3 and 6.31 mm respectively...

Present study was undertaken to investigate the influence of D-galactose binding lectin from Erythrina indica Lam. on the eggs and second instar larvae (64-72 hr) of melon fruit fly, Bactrocera cucurbitae (Coquillett). The lectin from E. indica seeds was extracted and purified by affinity chromatography using asilofetuin linked porous amino activated silica beads. The effects of various concent...