Accurate tumor excision is crucial in the locoregional treatment of cancer, and for this purpose, surgeons often rely on guide wires or radioactive markers for guidance toward the lesion. Further improvement may be obtained by adding optical guidance to currently used methods, in the form of intra-operative fluorescence imaging. To achieve such a multimodal approach, we have generated markers t...

PURPOSE To determine the roles of intercellular communication in embryonic eye growth and development, mice with a targeted deletion of the Cx43 gene were examined, and mice without both Cx43 and Cx50 were generated and analyzed. METHODS Embryonic eyes and lenses from wild-type mice, or mice deficient in Cx43, Cx50, or both Cx43 and Cx50 were collected and analyzed structurally by light and e...

Myoblast transfer therapy for Duchenne muscular dystrophy (DMD) largely fails due to cell death and inability of transplanted cells to engraft in diseased muscles. One method attempting to enrich for cell subpopulations is the Hoechst 33342 dye exclusion assay, yielding a side population (SP) thought to be progenitor enriched and a main population (MP). However, in vitro and transplant studies ...

The absence of stem cell-specific markers has posed challenges to the identification and isolation of stem cells. We report the isolation of a discrete and highly enriched population of neural stem cells from clonally derived colonies of neural stem cell and progenitor cells (neurospheres) after exposure to the fluorescent DNA binding dye Hoeschst 33342 and subsequent analysis via dual waveleng...

BACKGROUND This study was designed as a "proof-of-concept" to establish whether coronary computed tomographic angiography (CTA) has the capability to identify morphological features of plaque disruption. METHODS AND RESULTS In patients with unstable angina undergoing CTA and invasive coronary angiography within 30 days, quantitative CTA analysis was performed on all plaques for percent stenos...

introduction: basal cell carcinoma (bcc) is the most common cutaneous malignancy. proffered treatment for these lesions is surgery. based on patient’s age, drug allergies, and place of lesions, surgery may not be the best option, and alternative treatments should be considered. pulsed dye laser (pdl) may be a good alternative therapy. the aim of the present study was to determine the effectiven...

up to now, lots of materials such as active carbon, iron, manganese, zirconium, and metal oxides have been widely used for removal of dyes from contaminated water. among these, ferrite nanoparticle is an interesting magnetic material due to its moderate saturation magnetization, excellent chemical stability and mechanical hardness. graphene, a new class of 2d carbonaceous material with atom thi...