canine distemper virus (cdv) is the cause of canine distemper (cd) which is a severe and highly contagious disease in dogs. in the present study, a duplex reverse transcription polymerase chain reaction (rt-pcr) method was developed for the detection and differentiation of wild-type and vaccine strains of cdv. four primers were designed to detect and discriminate the two viruses by generating 6...

The use of the DNA duplex as a supramolecular scaffold is an established approach for the assembly of chromophore aggregates. In the absence of detailed structural insight, the characterization of thus assembled oligochromophores is, today, largely based on solution-phase spectroscopy. Here, we describe the crystal structures of three DNA-organized chromophore aggregates. DNA hybrids containing...

rapid and accurate identification of microorganisms have a significant impact on strategies and fish health management programs. hence, in this study a duplex pcr assay based on the 16s rrna gene for simultaneous detection of aeromonas hydrophila rticc 1032 and escherichia coli rticc 2325 from pure cultures, and challenged fish tissues was performed and their results were compared with the resu...

Duplexes are types of residential properties built and designed on two floors to accommodate a single family unit these have become trending form accommodation in recent times. An investor would be interested observing the trend capital value such investment able appreciate his venture. The aim this study is analyze duplex Benin property market, having objectives determining significance variat...

objective: duplex ultrasonography is a non-invasive, non-expensive screening test for carotid disease. intima media thickness (imt) is appropriate for atherosclerosis process diagnosis. leukoaraisis (la) increase the risk of cerebrovascular accident (cva). la patients need preventive medication for cva. imt is a non-invasive diagnostic and prognostic test in la. the aim of this study was evalua...

Advances in DNA nanotechnology have stimulated the search for simple motifs that can be used to control the properties of DNA nanostructures. One such motif, which has been used extensively in structures such as polyhedral cages, two-dimensional arrays, and ribbons, is a bulged duplex, that is, two helical segments that connect at a bulge loop. We use a coarse-grained model of DNA to characteri...

Two homochiral building blocks featuring a protected thiol, an alkene and a H-bond recognition unit (phenol or phosphine oxide) have been prepared. Iterative photochemical thiol-ene coupling reactions were used to synthesize oligomers containing 1-4 phosphine oxide and 1-4 phenol recognition sites. Length-complementary H-bond donor and H-bond acceptor oligomers were found to form stable duplexe...

Two homochiral building blocks featuring a protected thiol, an alkene and a H-bond recognition unit (phenol or phosphine oxide) have been prepared. Iterative photochemical thiol–ene coupling reactions were used to synthesize oligomers containing 1-4 phosphine oxide and 1-4 phenol recognition sites. Length-complementary H-bond donor and H-bond acceptor oligomers were found to form stable duplexe...

Canine distemper virus (CDV) is the cause of canine distemper (CD) which is a severe and highly contagious disease in dogs. In the present study, a duplex reverse transcription polymerase chain reaction (RT-PCR) method was developed for the detection and differentiation of wild-type and vaccine strains of CDV. Four primers were designed to detect and discriminate the two viruses by generating 6...

conclusions neither of studied variables was perfect to differentiate cirrhotic patients with and without evs. endoscopy is still the gold standard diagnostic method for diagnosing esophageal varices in patients with cirrhosis. it seems that some of the splenoportal doppler indices are promising, but more research and evaluation is necessary. results forty-four patients were male and 22 female....