نتایج جستجو برای: dotap

تعداد نتایج: 199  

Amiri Farahani M Barati F Dastoorani J, Tabandeh M

Background: Sperm mediated gene transfer was approved for producing transgenic animals. There are many studies which reported different success rate by this procedure. Failure in this project mostly is returned to transfection and stability of transferred genes. The other problem that may contribute in project failure may return to the testicular damages which occur at the time of transfection,...

Objective(s): Protection against leishmaniasis, in the murine model, is dependent on developing a potent CD4+ mediated Th1 type response. Liposomes can be applied as immunoadjuvants to stimulate immune responses to different antigens. In the present study, it was investigated whether DOTAP liposomes having SLA and imiquimod adjuvant, can induce a Th1 response and prote...

Introduction: In the present study, various formulations of cationic liposomes were designed and prepared using different cationic lipids. It was performed to assess the physicochemical properties, miRNA loading ability and cellular toxicity rates of liposomes in order to use in gene therapy. Methods: Different cationic liposome formulations (F1-F4) containing various cationic lipids, DOTAP, D...

2015
Jamie L. Betker Thomas J. Anchordoquy

Cationic reagents are commonly used to facilitate DNA delivery, and transfection experiments are typically initiated in cell culture where the optimal charge ratio is determined. While transfection rates are often enhanced at higher +/- charge ratios, the cellular toxicity associated with the greater amounts of cationic components at elevated charge ratios is often not considered. In addition, ...

Journal: :the iranian journal of pharmaceutical research 0
mostafa saffari department of pharmaceutics, school of pharmacy, shahid beheshti university of medical sciences, tehran, iran. farshad shirazi 1- department of pharmacology and toxicology, school of pharmacy, shahid beheshti university of medical, pharmaceutical research center, p.o.box 14155-3817, tehran, iran. 2- pharmaceutical sciences research center, shahid beheshti university of medical sciences, tehran, iran. mohammad ali oghabian research center for science and technology in medicine, tehran university of medical sciences, tehran, iran. hamid reza moghimi department of pharmaceutics, school of pharmacy, shahid beheshti university of medical sciences, tehran, iran.

the current methods for treatment of cancers are inadequate and more specific methods such as gene therapy are in progress. among different vehicles, cationic liposomes are frequently used for delivery of genetic material. this investigation aims to prepare and optimize dotap cationic liposomes containing an antisense oligonuclotide (asodn) against protein kinase c alpha in non-small cells lung...

Journal: :iranian journal of basic medical sciences 0
mohammad ramezani pharmaceutical research centre, school of pharmacy, mashhad university of medical sciences, mashhad, iran bizhan malaekeh-nikouei nanotechnology research centre, school of pharmacy, mashhad university of medical sciences, mashhad, iran tahreh khakshoor nanotechnology research centre, school of pharmacy, mashhad university of medical sciences, mashhad, iran mohammad malaekeh-nikouei pharmaceutical research centre, mashhad university of medical sciences, mashhad, iran

the development of efficient and safe carrier system to transfer dna into cells is essential in non-viral gene therapy. the aim of the present study was to evaluate the effect of linear polyetheneimine (lpei) (2500 da) on the physicochemical and biological properties of lipopolyplexes constructed from liposomes and lpei. materials and methods different lipopolymers were synthesized from lpei an...

برزگری فیروزآبادی, فاطمه, جاوید, آمنه, شیخ ها, محمد حسن, عریان, شهربانو, کلانتر, سید مهدی,

Introdution: The aim of this study was to investigate the physico-chemical properties, surface charge and cytotoxicity of nanocomposite formulations for the design of lipid nano-systems with maximum loading for nucleic acids in bone marrow cancer. Methods For this study, six types of liposomes with different compositions were synthesized using DPPC, cholesterol, DSPE-mPEG (2000) and DOTAP. The...

2001
A. N. Uduehi U. Stammberger S. Frese R. A. Schmid

Objective: Transient expression of therapeutic genes within lung allografts may modulate the pathological processes following allotransplantation. Whilst ef®cient gene transfer to lungs has been reported with viral vectors, their usefulness is limited on the grounds of safety. Since non-viral systems overcome many of these safety issues, our studies were designed to evaluate the ef®ciency of se...

Journal: :The Analyst 2014
Mohanlal Bhuvana Venkataraman Dharuman

Construction of spherical liposomes is critical for developing tools for targeted gene and drug delivery applications in biotechnology and medicine, however, it has been demonstrated only in solution phase until now. Spherical liposome tethering on pristine thiol monolayer on gold transducer and its application to label free DNA sensing and transfection has rarely been reported. Here, we report...

Journal: :The Journal of biological chemistry 2005
Bouna-Moussa Tandia Caroline Lonez Michel Vandenbranden Jean-Marie Ruysschaert Abdelatif Elouahabi

It has been previously shown that transfection activity of cationic liposome/DNA lipoplexes delivered systemically is drastically inhibited by lipoproteins (Tandia, B. M., Vandenbranden, M., Wattiez, R., Lakhdar, Z., Ruysschaert, J. M., and Elouahabi, A. (2003) Mol Ther. 8, 264-273). In this work, we have compared the binding/uptake and transfection activities of DOTAP (N-[1-(2,3-dioleoyloxy)pr...

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