Five different immunoassay formats were examined for their ability to detect a minute quantity of prey remains in predator guts. The convergent lady beetle, Hippodamia convergens Guérin-Meneville, that had consumed either one or five pink bollworm, Pectinophora gossypiella (Saunders), eggs was evaluated by the following immunoassays: three variations of enzyme-linked immunosorbent assay (ELISA)...

Snake bite cases in Malaysia most often involve the species Naja-naja and Calloselasma rhodostoma. In keeping with the need for a rapid snake venom detection kit in a clinical setting, plate and dot-ELISA test for the venoms of Naja-naja sumatrana, Calloselasma rhodostoma and the cobra venom fraction V antigen was developed. Polyclonal antibodies were raised and further used to prepare the reag...

Abstract BACKGROUND: Diagnosis of Echinococcus granulosus in the definitive host particularly in dog is the significant complication in the endemic area. OBJECTIVES: The aim of this study is serological detection of E. granulosus in the infected dogs. METHODS: Dot-ELISA based on the copro-antigen and recombinant EPC1 antigen (rEPC1) for antibody detection was performed. Blood and fecal samples ...

Plant diseases caused by viruses are the most menace to sustainable agriculture, leading numerous billion dollars in losses annually. Proper and accurate detection of plant is always key developing appropriate solutions manage economic them. Advances technology have simplified tools available detect diagnose at a time when control measures should be used. Currently, nucleic acid serological bas...

Giardia lamblia is a frequent pathogen in persistent diarrhoea. Both humoral and cellular immune responses are important in clearing the parasite and providing immunity. A rapid sensitive and specific serological test like dot ELISA could be useful for early detection of Giardia specific serum antibodies for routine diagnosis. This study reports the use of dot ELISA for the detection of Giardia...

Immunological assays such as CIE, LA, and Dot-ELISA were compared in order to diagnose community-acquired pneumonia. Serum, pleural fluid and urine samples were comparatively employed for bacterial antigen detection. Dot-ELISA proved to be an original and practical alternative procedure for detecting bacterial polysaccharide antigens from pleural fluid and/or concentrated urine samples, providi...

The aim of the present study was to standardize and evaluate dot-Enzyme linked immunosorbent assay (Dot-ELISA), a simple and rapid test for the detection of cysticercus antibodies in the serum for the diagnosis of neurocysticercosis (NCC). The antigen used in the study was a complete homogenate of Cysticercus cellulosae cysts obtained from infected pigs and dotted on to nitrocellulose membrane....

A simple dot blot test with diacyltrehalose (DAT) as the antigen was developed to detect anti-DAT antibodies in tuberculous patients. To enhance antigen-antibody reaction detection, rabbit serum raised against human immunoglobulins was used prior to incubation with a protein A-colloidal gold complex. With the dot blot system, it was possible to obtain a sensitivity similar to that of enzyme-lin...