Background: ATP-binding cassette membrane transporter G2 (ABCG2) gene is one of transporter family and well characterized for their association with chemoresistance. Promoter methylation is a mechanism for regulation of gene expression. O6-Methyl guanine DNA methyl transferase (MGMT) gene plays a fundamental role in DNA repair. MGMT has the ability to remove alkyl adducts from DNA at the O6 pos...

Recent findings on the genomic heterogeneity of mammalian DNA repair have disclosed new features about molecular aspects of the repair processes and have also raised new interesting questions to help direct our research. The studies on preferential DNA repair in active genes demonstrate that there are variations in the repair processes corresponding to different parts of the genome and we are n...

Modified single-stranded DNA oligonucleotides can direct the repair of genetic mutations in yeast, plant and mammalian cells. The mechanism by which these molecules exert their effect is being elucidated, but the first phase is likely to involve the homologous alignment of the single strand with its complementary sequence in the target gene. In this study, we establish the importance of such DN...

We have studied DNA repair in the Chinese Hamster Ovary (CHO) metallothionein (MT) gene after UV-light induced damage. The repair was examined comparatively with or without transcriptional activation of the gene by incubation in the presence of the heavy metal ZnCl2. Whereas the repair efficiency was very low in the uninduced state, it increased significantly after induction of the gene. The pr...

The DNA-dependent protein kinase catalytic subunit (DNA-PKcs) is a encoded by the PRKDC gene in humans and plays crucial role repairing DNA double-strand breaks (DSBs). Recent studies have revealed that DNA-PKcs has additional functions cell beyond DSB repair, including transcriptional regulation, telomere protection capping, preserving chromosomal integrity, regulating senescence, apoptosis, a...

BACKGROUND Radiotherapy-induced DNA double-strand breaks (DSBs) are critical cytotoxic lesions. Inherited defects in DNA DSB repair pathways lead to hypersensitivity to ionising radiation, immunodeficiency and increased cancer incidence. A patient with xeroderma pigmentosum complementation group C, with a scalp angiosarcoma, exhibited dramatic clinical radiosensitivity following radiotherapy, r...

Oxidative DNA damage is one of the earliest detectable events in several neurodegenerative diseases, often preceding the onset of the clinical symptoms. Moreover, neurons in the adult human brain can re-enter the cell division cycle, likely allowing DNA repair. Impairments of DNA repair pathways are reported in neurons of patients suffering from one of several neurodegenative diseases and might...

Prostate cancer is histologically and molecularly heterogeneous. Clinically significant disease is often driven by dominant intra-prostatic lesions (IPLs). Prostate cancers cluster into molecular phenotypes with substantial genetic heterogeneity making pathway-based molecular analysis appealing. MRI/ultrasound fusion biopsy provides a unique opportunity to characterize tumor biology of discrete...

Gene 32 of phage T4 has been shown previously to be involved in recombinational repair of UV damages but, based on a mutant study, was thought not to be required for excision repair. However, a comparison of UV-inactivation curves of several gene 32 mutants grown under conditions permissive for progeny production in wild-type or polA- hosts demonstrates that gene 32 participates in both kinds o...

The nuclear pore complex is a multiprotein comprising approximately 30 different proteins called nucleoporins (Nups). Recent studies in yeast, animals, and plants have shown that the Nups bind to chromatin interact with transcription factors, chromatin-remodeling histone-modifying enzymes, structural proteins, including CCCTC-binding factor cohesin. These findings suggest provide subnuclear pla...