The performances of the Etest and the disk diffusion methods for testing of the susceptibilities of 235 Candida glabrata isolates to fluconazole and voriconazole were compared with that of the National Committee for Clinical Laboratory Standards (NCCLS) approved standard broth microdilution (BMD) method. The NCCLS method used RPMI 1640 broth medium, and MICs were read after incubation for 48 h ...

The agar dilution method has been standardized by the CLSI for the susceptibility testing of Campylobacter species, and according to these standards, the disk diffusion method should be used only in screening for macrolide and ciprofloxacin resistance. Nevertheless, the disk diffusion test is currently widely used, since it is easy to perform in clinical microbiology laboratories. In this study...

21 The agar dilution method has been standardized by the CLSI for the susceptibility testing of 22 Campylobacter species, and according to these standards, the disk diffusion method should only 23 be used for the screening of macrolide and ciprofloxacin resistance. Nevertheless, the disk 24 diffusion test is currently widely used, since it is easy to perform in clinical microbiology 25 laborato...

Mannitol salt agar was evaluated for detection of oxacillin resistance in 136 Staphylococcus aureus isolates. All mecA-positive isolates (n = 54) were correctly categorized as oxacillin resistant by the disk diffusion test (1-microgram disk; zone diameter, < 16 mm); the specificity was 97.6%. Agar screening (2 micrograms of oxacillin per ml) revealed a sensitivity of 98.1% and a specificity of ...

The aim of this study was to investigate the carriage of vancomycin-resistance genes by Staphylococcus aureus found in drinking water supplies. A total of 100 samples (potable water and faucet swabs) were analyzed for the presence of S. aureus and their carriage of vancomycin-resistance genes. Mannitol salt agar was used for the isolation of staphylococci, and confirmation of S. aureus was carr...

Forty-seven Bordetella pertussis isolates recovered from January 1985 to June 1997 at Primary Children's Medical Center were tested for erythromycin resistance. Agar dilution MICs were determined on Regan-Lowe agar. Forty-six isolates were found to be erythromycin susceptible (all MICs were less than or equal to 0.12 microg/ml). One isolate was found to be erythromycin resistant (MIC, 32 microg...

Rifampin was incorporated into Middlebrook 7H10 medium either by adding an aliquot of the antibiotic into melted agar (final concentration 1.0 and 3.0 mug/ml) or by submerging a 5 or 15 mug of rifampin paper disk into 5 ml of melted agar contained in one quadrant of a Felson "X" plate. At intervals, plugs of agar were removed from the stored plates and assayed. Plates stored at 5 C for 28 days ...

background and objectives: antibacterial susceptibility testing of clinical bacterial isolates through disk diffusion method plays a major role in antibacterial treatment. one of the main factors affecting the result of these tests is the type, structure and quality of the disks. the main objective of this study was to compare the agreement of antibiotic disks originated from three companies on...

Introduction: In this study, using the phenotypic and genotypic methods, oxacillin susceptibility in Staphylococcus aureus (S. aureus) strains isolated from patients at two government hospitals in Ilam, Iran was tested. Materials and methods: Out of 200 S. aureus isolates from different human clinical specimens consisting of blood (31%), wound (20%), urine (21%), catheters (7%), sputum (12...