Membrane diafiltration process was developed in order to fractionate the water soluble proteins of supernatant obtained after Spirulina bead milling disruption and centrifugation. The crude extract filtered with 50 kDa, 150 300 kDa or 0.2 µm membranes. Phycocyanin purified extracts were produced filtration on membrane allowed high recovery (more than 89%) A C into retentate permeate as well sep...

Ingestion of even small amounts of MDMA ('ecstasy') by a small subset of the population may result in a potentially fatal clinical syndrome of severe hyperpyrexia, cardiovascular collapse, coagulopathy, rhabdomyolysis and multiple organ failure. Rapid and aggressive temperature control is of utmost importance in the management of these patients. We report a case of MDMA toxicity presenting with...

Several methods for the preparation of "white ghosts" or "resealed ghosts" were described in the recent literature. This article compares three methods to prepare white, resealed ghosts from bovine erythrocytes based on the principle of hypotonic lysis. The methods described differ by the removal of hemoglobin from the empty cells. The main difference between the standard centrifugation, the ge...

This paper deals with the time-optimal operation and parameter estimation problem of a general diafiltration process in the presence of fouling. Fouling stands for one of the dominant problems in the membrane separation processes. The dynamic behavior of the fouled membrane is described by a general fouling model taken from literature. An Extended Kalman filter is proposed for the recursive est...

membranes to completely remove, replace, or lower the concentration of salts or solvents from solutions containing proteins, peptides, nucleic acids, and other biomolecules. The process selectively utilizes permeable (porous) membrane filters to separate the components of solutions and suspensions based on their molecular size. An ultrafiltration membrane retains molecules that are larger than ...

PURPOSE Membrane-based Tangential Flow Filtration (TFF) unit operations are used for clarifying, concentrating, and purifying proteins. This technical brief is a practical introduction to protein processing using tangential flow filtration. It is intended to help scientists and engineers achieve their protein processing objectives by discussing how the choice of key components and operating par...