The effects of eritadenine, a constituent of the Lentinus edodes mushroom, and ethanolamine, the base constituent of phosphatidylethanolamine (PE), on fatty acid desaturase activities and lipid profiles were investigated comparatively in rats. Rats were fed a control diet or a diet supplemented with either eritadenine (0.05 g/kg) or ethanolamine (8 g/kg) for 14 d. Eritadenine and ethanolamine h...

Ethanolamine kinase (ATP:ethanolamine O-phosphotransferase, EC 2.7.1. 82) catalyzes the committed step of phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway. The gene encoding ethanolamine kinase (EKI1) was identified from the Saccharomyces Genome Data Base (locus YDR147W) based on its homology to the Saccharomyces cerevisiae CKI1-encoded choline kinase, which also exhibits eth...

Di-ethanolamine (DEA) is a well-known environmental pollutant used in manufacturing soap, detergent, body lotion, and other sanitary products. DEA has been reported to cause cytotoxicity in different tissue and cell, but no study was found to explain the toxic effect of DEA on rat bone marrow mesenchymal stem cells (BMSCs) differentiation. Thus in the present study, the differentiation property...

After the original demonstration by Stetten (1) that the nitrogen atom of DL-serine-N15 is converted to the nitrogen of ethanolamine in the intact rat, considerable evidence has accrued to indicate that in mammalian tissues L-serine is decarbosylated to ethanolamine. Not only are the B-carbon, B-hydrogen, and nitrogen of serine extensively utilized as a unit for the synthesis of the ethanolamin...

The conditions for maximal activity of ethanolamine phosphokinase from rat liver were determined. All of the activity was located in the supernatant fraction after centrifugation at Ioo ooo X g for 6o min. The enzyme activity had a pH opt imum at 8.5 and an apparent I(m for ethanolamine of I , IO 4 M at an ATP and Mg 2+ concentration of 3.o mM. The enzyme uses the Mg-ATP complex as substrate an...

Phosphatidyl ethanolamine and lipopolysaccharide were extracted and purified from the cell envelope fractions of Escherichia coli and Salmonella typhimurium. The two components were studied separately and after recombination, by use of electron microscopy and monolayer techniques, and by measuring their ability to participate in the enzyme-catalyzed uridine diphosphate-galactose:lipopolysacchar...

Cultured dissociated cells from rat embryo cerebral hemisphere iqcorporate [gH]and [U-14C]ethanolamine into cellular lipids. Nearly all radioactivity in the lipid fractions is incorporated into 1,2diacylethanolamine phosphoglycerides and I-alkenyl,P-acylethanolamine phosphoglycerides (plasmalogen). Kinetic data suggest that the rate of labeling of both ethanolamine phospholipids from the phosph...

A method for the separation and quantitative estimation of ethanolamine, dimethyl ethanolamine, and monomethyl ethanolamine derived from lipids is described. After acid hydrolysis of the lipids, the bases are extracted from the neutralized hydrolysate with t-butonol and separated by gas-liquid chromatography.

Among the more commonly recognized constituents of naturally occurring phospholipides are phosphatidyl ethanolamine and phosphatidyl serine. The most feasible analytical procedures for these components have been based on the determination of ethanolamine and serine in a hydrolysate of the lipide. In general, assays for serine and ethanolamine have consisted of three main steps: (a) hydrolysis o...

The role of serine as a precursor and metabolic regulator for phosphatidylethanolamine biosynthesis in the hamster heart was investigated. Hearts were perfused with 60 p~ [l-SH]ethanolamine in the presence or absence of serine for up to 60 min. Ethanolamine uptake was attenuated by 0.05-10 mM serine in a noncompetitive manner, and the incorporation of labeled ethanolamine into phosphatidylet...