Detergent-soluble DNA is the fraction (2-4%) of DNA that is released into the supernate upon mild detergent lysis. It is nonmitochondrial in origin. It labels efficiently with deoxy[3H]ribonucleosides and the labeling is prevented by inhibitors of polymerase alpha and ribonucleotide reductase. In previous publications we have characterized detergent-soluble DNA from splenocytes of immunological...

This study was conducted to investigate the effect of biological treatment withPleurotus floridafungi on chemical composition and rumen dry matter (DM) and organic matter (OM) degradability of wheat and barley straw. Wheat and barley straw were collected from Golestan province of Iran and after pasteurization mixed with fungi spawn (than 5% by weight). After 21 days samples were dried in oven (...

this study was conducted to investigate the effect of biological treatment withpleurotus floridafungi on chemical composition and rumen dry matter (dm) and organic matter (om) degradability of wheat and barley straw. wheat and barley straw were collected from golestan province of iran and after pasteurization mixed with fungi spawn (than 5% by weight). after 21 days samples were dried in oven (...

The infective dauer juvenile (DJ2) of Anguina agrostis, a stage capable of surviving desiccation, is up to sixfold more resistant to the detergent sodium dodecyl sulfate than are freshly hatched juveniles or adult males, and twofold more resistant to the anesthetic phenoxypropanol. Thus, the DJ2, like dauer stages of other species, may also be more resistant to various types of environmental st...

The r\l=o^\leof acrosomal enzymes of mammalian spermatozoa in sperm penetration through the investments of the ovum and subsequent fertilization has been studied since the development of the method based on treatment ofspermatozoa with the cationic detergent, Hyamine, for detachment of acrosomes (Hartree & Srivastava, 1965). Srivastava, Zaneveld & Williams (1970) modified this method, using Tri...

The biodegradability of the principal component of synthetic detergent products known as linear alkylbenzene sulphonate (LAS) has been contentious, hence the need to evaluate its primary biodegradation by indigenous microorganisms in wastewater ecosystem. The native microbial consortium of a wastewater ecosystem found to utilize detergent components were characterized using standard and convent...

After exposure of segments of rabbit taenia coli to the nonionic detergent Triton X-100, tension could be induced by increasing the [Ca2+] in the micromolar range. In the presence of a saturating [Ca2+], this preparation developed nearly 100% of the control tetanus tension recorded from the intact muscle prior to the detergent treatment. In addition, tension could be induced by increasing the [...

Mild detergent treatment (0.1% Sarkosyl-0.1% beta-mercaptoethanol) of Dane particle-rich fraction from human serum resulted in the release of core particles together with HBe antigen activity when examined by the reversed passive haemagglutination method. Furthermore, when the core particles isolated by the above procedure were exposed to stronger detergent (1% Sarkosyl-0.1% beta-mercaptoethano...

A diverse set of cell surface eukaryotic proteins including receptors, enzymes, and adhesion molecules have a glycosylphosphoinositol-lipid (GPI) modification at the carboxy-terminal end that serves as their sole means of membrane anchoring. These GPI-anchored proteins are poorly solubilized in nonionic detergent such as Triton X-100. In addition these detergent-insoluble complexes from plasma ...