نتایج جستجو برای: dependent probe amplifi cation
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Growth hormone, a protein hormone, has a remarkable role in growth, reproduction and milk production. The present investigation was aimed at studying the genetic variation in growth hormone gene locus 4th exon, 4th intron and 5th exon in Murrah buffalo bulls by PCR-RFLP. The growth hormone gene was amplifi ed by PCR using oligonucleotide primers standardized for Bos taurus species. A 428 bp fra...
آنزیم های dna ligase پایدار به حرارت به علت قابلیت های خود از اهمیت بالایی در زیست شناسی مولکولی برخوردارند. در این بین، آنزیم dna ligase از باکتری thermus thermophilus (tth dna ligase) با قابلیت های ویژه ی خود من جمله پایداری بالای حرارتی و دقت بالای عملکرد، می تواند کاربردهای وسیعی را در تکنیک های تشخیصی بیماری های ژنتیکی مانند تکنیک lcr (ligation chain reaction) و mlpa (multiple ligation-dep...
The amplifi cation refractory mutation system (ARMS), which has also been described as allele-specifi c PCR (ASP) and PCR amplifi cation of specifi c alleles (PASA), is a PCR-based method of detecting single base mutations (Newton et al., 1989). ARMS has been applied successfully to the analysis of a wide range of polymorphisms, germline mutations and somatic mutations. The technique has the ab...
Primer The steam drill was on the right hand side, John Henry was on the left, Says before I let this steam drill beat me down, I'll hammer myself to death " —The Ballad of John Henry (American, traditional) O rganisms and molecules achieve evolutionary success via many possible paths, what evolutionary biologists sometimes call the " tempo and mode " of evolution [1]. In contrast, engineered m...
a relationship based on the modified couple stress theory is developed to investigate the flexural sensitivity of an atomic force microscope (afm) with assembled cantilever probe (acp). this acp comprises a horizontal cantilever, two vertical extensions and two tips located at the free ends of the extensions which form a caliper. an approximate solution to the flexural vibration problem is obta...
Neuroblastoma is an embryonic neoplasm of the sympathetic nervous system that accounts for approximately 15 percent of all pediatric cancer fatalities. Genomic change in neuroblastoma has been shown to correlate with both behavior and outcome. More specifi cally, amplifi cation of the MYCN oncogene occurs in about 20% of neuroblastoma patients and has been shown to provide important prognostic ...
We present a case report of a complete response to a combination of trastuzumab and pegylated liposomal doxorubicin in a female patient with recurrent metastatic HER-2 positive breast cancer after anthracy-cline-based adjuvant primary chemotherapy. To our knowledge this sequence of events has not previously been reported. In September 2003, after routine mammographic detection, this 59-year-old...
Primary brain tumors of glial origin often overexpress epidermal growth factor receptors (EGF-Rs). This may be associated with amplifi cation of the EGF-R gene. We have examined tissue from 23 glioblastoma multiforme tumors and found amplification and rearrangement of the EGF-R gene in four of these. The cytoplasmic domain of the EGF-R gene was invariably amplified in these four tumors, while t...
Authors’ Affi liation: Department of Pathology, Memorial Sloan-Kettering Cancer Center, New York, New York Corresponding Author: Jorge S. Reis-Filho, Department of Pathology, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10065. Phone: 212-639-8054; Fax: 212-772-8521; E-mail: reisfi lj@ mskcc.org doi: 10.1158/2159-8290.CD-12-0585 ©2012 American Association for Cancer Res...
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