Dendritic cells play a key role in determining adaptive immunity, and there is growing interest in characterizing and manipulating the interactions between dendritic cells and biomaterial surfaces. Contact with several common biomaterials can induce the maturation of immature dendritic cells, but substrates that reduce dendritic cell maturation are of particular interest within the field of cel...

Dendritic cells produce IL-12 both in response to microbial stimuli and to T cells, and can thus skew T cell reactivity toward a Th1 pattern. We investigated the capacity of dendritic cells to elaborate IL-12 with special regard to their state of maturation, different maturation stimuli, and its regulation by Th1/Th2-influencing cytokines. Monocyte-derived dendritic cells were generated with GM...

Background: Generation of monocyte-derived dendritic cells (MDDC) is induced in the presence of GM-CSF and IL-4, and a maturation stimulus is added to the monocyte culture to obtain mature Dendritic Cells (DCs) suitable for therapy. TNF-α is the most common cytokine used for activating DCs and generating mature MDDC either alone or in combination with other cytokines. Objective: To compare effe...

background: fusion of dendritic cells (dcs) with melanoma cells could reinforce the antigenicity of tumors as a strategy for the treatment of malignant melanoma. however, the insufficient quantity of dcs and the low fusion efficiency limits the development of such approach. objective: to define the dosage of the stimulating factors as well as the induction condition for the optimal dcs preparat...

Inflammatory mediators in the tumour microenvironment promote tumour growth, vascular development and enable evasion of anti-tumour immune responses, by disabling infiltrating dendritic cells. However, the constituents of the tumour microenvironment that directly influence dendritic cell maturation and function are not well characterised. Our aim was to identify tumour-associated inflammatory m...

OBJECTIVE Although dendritic cells are known to play a role in atherosclerosis, few studies have examined the contribution of the wide variety of dendritic cell subsets. Accordingly, their roles in atherogenesis remain largely unknown. We investigated the ability of different dendritic cell subsets to become foam cells after contact with aggregated low-density lipoprotein (LDL; the predominant ...

background: generation of an effective dendritic cell (dc) based cancer vaccine depends on appropriate differentiation of monocytes in vitro. objective: to compare the effects of monocyte separation methods, flask adherence (flask-dc) and magnetic activated cell sorting (macs-dc), on phenotypic and functional characteristics of resultant dcs. methods: dcs from healthy volunteers were generated ...

background & objectives: chronicity of lupus nephritis (ln) should be considered for interaction of cell mediated immunity (cmi) and dendritic cells in glomeruli and tubulointerstitial areas. in this study establishment of immunohistopathological changes of dendritic cells and other immune effector cells in lupus nephritis comparing with non-lupus nephritis was performed. materials & m...