Background: Dendritic cells (DC) are a key regulator of the immune response, and interferon- beta (IFN-β) is considered an immunomodulatory molecule for DC. Objective: The purpose of this study was to evaluate the ability of IFN-β treated DC to induce cytokine secretion by CD4+ T cells. Methods: Dendritic cells were generated from blood monocytes with granulocyte-monocyte colony-stimulating fac...

background: dendritic cells (dc) induce tumor or pathogen-specific t cell responses in humans. several laboratories have developed culture systems, including maturation factors for human dc from peripheral blood monocytes. we comprehensively compared standard maturation stimulus, an autologous monocyte-conditioned medium (mcm), with heparin for their ability to promote uniformly mature dc that ...

the wharton’s jelly of the umbilical cord is believed to be a source of mesenchymal stem cells (mscs) which can be therapeutically applied in degenerative diseases. in this study, we investigated the immunomodulatory effect of umbilical cord derived- mesenchymal stem  cells (uc-mscs) and  bone  marrow-derived-mesenchymal stem  cells (bm-mscs) on differentiation, maturation, and endocytosis of m...

back ground: candida albicans is a member of the normal human microflora. c. albicans cell wall is composed of several protein and carbohydrate components which have been shown to play a crucial role in c. albicans interaction with the host immune system. major components of c. albican cell wall are carbohydrates such as mannans, β glucans and chitins, and proteins that partially modulate the h...

background: the immunomagnetic separation technique is the basis of monocyte isolation and further generation of monocyte-derived dendritic cells. objective: to compare the efficiency of monocyte positive and negative separation, concentration of beads, and their impact on generated dendritic cells. methods: monocytes were obtained using monoclonal antibody-coated magnetic beads followed the fi...

results there was a significant difference in prevalence of ebv presence between patients and controls. the ebv was found by rt-pcr in 19 (63.33%) out of 30 patients and only in eight (26.7%) of 30 control samples. the p = 0.004, was calculated using chi-square test (or: 4.75; 95% ci: 1.58 ‒ 14.25). conclusions our study is the first investigation performed on patients with lch and its possible...

Dendritic cells (DCs) induce pathogen-specific T cell responses. We comprehensively studied the effects of addition of maturation stimulus, fibroblasts (fibroblast conditioned medium), PHA activated T cells (T cell conditioned medium), and mixture of fibroblast & PHA activated T cells (FCM-TCCM) conditioned media on maturation of DCs. Monocytes were cultured with GM-CSF and IL-4 for five days. ...

objective: dendritic cells have a critical role in control and regulation of immune responses. it is believed that these cells can be used for the treatment of many diseases. one of the methods used in immunotherapy is based on generating of tolerogenic dendritic cells through inhibition of expression costimulatory molecules. cd40 is one of the costimulatory molecules, and inhibition of express...