نتایج جستجو برای: decellularization

تعداد نتایج: 565  

Journal: :European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery 2012
Hong-Gook Lim Soo Hwan Kim Sun Young Choi Yong Jin Kim

OBJECTIVE Calcification plays a major role in the failure of bioprosthetic and other tissue heart valve substitutes. The objective of this study was to evaluate the anticalcification effect of decellularization and detoxification in glutaraldehyde (GA)/genipin- and solvent-fixed bovine pericardium using a rabbit intramuscular model which is effective for assessing calcification in bioprosthetic...

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2005
Sylvain Roy Paolo Silacci Nikolaos Stergiopulos

Roy, Sylvain, Paolo Silacci, and Nikolaos Stergiopulos. Biomechanical proprieties of decellularized porcine common carotid arteries. Am J Physiol Heart Circ Physiol 289: H1567–H1576, 2005. First published May 20, 2005; doi:10.1152/ajpheart.00564.2004.—To analyze the effects of decellularization on the biomechanical properties of porcine common carotid arteries, decellularization was performed b...

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Journal: :Journal of Tissue Engineering 2021

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2016
Catherine A Bautista Hee Jun Park Courtney M Mazur Roy K Aaron Bahar Bilgen

Articular cartilage has a limited capacity to heal itself and thus focal defects often result in the development of osteoarthritis. Current cartilage tissue engineering strategies seek to regenerate injured tissue by creating scaffolds that aim to mimic the unique structure and composition of native articular cartilage. Decellularization is a novel strategy that aims to preserve the bioactive f...

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2013
Panagiotis Maghsoudlou Fanourios Georgiades Athanasios Tyraskis Giorgia Totonelli Stavros P. Loukogeorgakis Giuseppe Orlando Panicos Shangaris Peggy Lange Jean-Marie Delalande Alan J. Burns Angelo Cenedese Neil J. Sebire Mark Turmaine Brogan N. Guest John F. Alcorn Anthony Atala Martin A. Birchall Martin J. Elliott Simon Eaton Agostino Pierro Thomas W. Gilbert Paolo De Coppi

Tissue engineering of autologous lung tissue aims to become a therapeutic alternative to transplantation. Efforts published so far in creating scaffolds have used harsh decellularization techniques that damage the extracellular matrix (ECM), deplete its components and take up to 5 weeks to perform. The aim of this study was to create a lung natural acellular scaffold using a method that will re...

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2013
Panagiotis Maghsoudlou Giorgia Totonelli Stavros P Loukogeorgakis Simon Eaton Paolo De Coppi

Successful tissue engineering involves the combination of scaffolds with appropriate cells in vitro or in vivo. Scaffolds may be synthetic, naturally-derived or derived from tissues/organs. The latter are obtained using a technique called decellularization. Decellularization may involve a combination of physical, chemical, and enzymatic methods. The goal of this technique is to remove all cellu...

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2013
Hana Hrebikova Daniel Diaz Jaroslav Mokry

Background. A biological scaffold from extracellular matrix can be produced by a variety of decellularization methods whose caveat consists in efficiently eliminating cells from the treated tissue. This scaffold can be used in diverse applications for tissue engineering and organ regeneration. Preservation of the extracellular matrix ultrastructure is highly desirable because of its unique arch...

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2016
Pedro L Sánchez Mª Eugenia Fernández-Santos Mª Angeles Espinosa Mª Angeles González-Nicolas Judith R Acebes Salvatore Costanza Isabel Moscoso Hugo Rodríguez Julio García Jesús Romero Stefan M Kren Javier Bermejo Raquel Yotti Candelas Pérez del Villar Ricardo Sanz-Ruiz Jaime Elizaga Doris A Taylor Francisco Fernández-Avilés

Perfusion decellularization of cadaveric hearts removes cells and generates a cell-free extracellular matrix scaffold containing acellular vascular conduits, which are theoretically sufficient to perfuse and support tissue-engineered heart constructs. This article contains additional data of our experience decellularizing and testing structural integrity and composition of a large series of hum...

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2017
Erik Elebring Vijay K Kuna Niclas Kvarnström Suchitra Sumitran-Holgersson

Despite progress in the field of decellularization and recellularization, the outcome for pancreas has not been adequate. This might be due to the challenging dual nature of pancreas with both endocrine and exocrine tissues. We aimed to develop a novel and efficient cold-perfusion method for decellularization of porcine pancreas and recellularize acellular scaffolds with human fetal pancreatic ...

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Journal: :PloS one 2016
Pietro Maria Ferrando Davide Balmativola Irene Cambieri Maria Stella Scalzo Massimiliano Bergallo Laura Annaratone Stefania Casarin Mara Fumagalli Maurizio Stella Anna Sapino Carlotta Castagnoli

Human Acellular Dermal Matrices (HADM) are employed in various reconstructive surgery procedures as scaffolds for autologous tissue regeneration. The aim of this project was to develop a new type of HADM for clinical use, composed of glycerolized reticular dermis decellularized through incubation and tilting in Dulbecco's Modified Eagle's Medium (DMEM). This manufacturing method was compared wi...

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