The routine study of bone marrow trephine biopsies involves fixation, decalcification, paraffin-embedment, sectioning and staining. However, this process creates artifacts, produces shrinkage of tissue, consumes time and can result in sections of unsatisfactory cytological quality. It also renders the tissue unsuitable for enzyme-histochemical and immunohistochemical analyses. Frozen section of...

background. many types of toothpastes contain substances that can remineralize initial enamel caries. this study aimed to assess the effect of nano-hydroxyapatite (nha) on microhardness of artificially created carious lesions. methods. in this in vitro study, nha was prepared using sol-gel technique and added to the toothpaste with 7% concentration. a total of 80 extracted sound teeth were coll...

introduction:visualization of inner ear structures is a valuable strategy for researchers and clinicians working on hearing pathologies. optical coherence tomography (oct) is a high-resolution imaging technology which may be used for the visualization of tissues. in this experimental study we aimed to evaluate inner ear anatomy in well-prepared human labyrinthine bones.materials and methods:thr...

The aim of this study is to compare the efficiency of radiopaque dye, decalcification, and histological sectioning in detection of accessory canals in mandibular 1 st molars. 30 Extracted mandibular 1 st molars were selected. The teeth selected for the study were stored in normal saline. Endodontic access openings were made using a round bur and pulp tissue was removed from the pulp chamber usi...

A ny successful program of preventive dentistry for orthodontic patients must be based upon the realization that these patients represent an advanced caries risk.l One may argue at length as to the specific causes of decalcification frequently encountered in patients over long periods of banding, and there are differences of opinion as to the progression or regression of these lesions after the...

This is the second paper on principles of demineralization. The initial paper is dedicated to the common definitions and the history of demineralization. In present work we review the principles and mechanisms of decalcification, i.e., removing the mineral Ca-containing compounds (phosphates and carbonates) from the organic matrix in its two main aspects: natural and artificial. Natural chemica...