Polymerase chain reaction (PCR) has been proposed as a method to identify bacteria in clinical samples because it is more sensitive than culture techniques and can produce results rapidly. However, PCR can detect DNA from dead cells and thus cannot distinguish between live and dead cells in a tissue sample. Killed Staphylococcus aureus cells were implanted into the femurs and knee joints of rat...

introduction: different environmental factors such as drugs, ionizing radiation, etc can induce apoptosis.in the present study the effects of dose- rate and post-irradiation incubation time on gamma radiation inducedapoptosis in human breast carcinoma cell line (t-47d) (invasive ductal carcinoma) was investigated. material and methods: the t-47d cells were cultured in t25 flasks and during log ...

Background: Ischemic stroke, as a health problem caused by the reduced blood supply to the brain, can lead to the neuronal death. The number of reliable therapies for stroke is limited. Mesenchymal stem cells (MSCs) exhibit therapeutic achievement. A major limitation of MSC application in cell therapy is the short survival span. MSCs affect target tissues through the secretion of many paracrine...

Effluents from well-acclimated microbial fuel cells (MFCs) have been widely used as inocula to start up new MFC reactors. However, the actual cell concentrations and viability of exoelectrogens in these effluents not well examined. In this study, effluent acetate- or wastewater-fed MFCs were examined using a quantitative polymerase chain reaction (qPCR) method specific for Geobacter spp. that a...

It was observed that Freundlich's law is obeyed when methylene blue is adsorbed from different aqueous solutions by mixtures of dead and live bacterial cells. A simple and precise colorimetric method was developed for determining the percentage of dead cells.

introduction: infecting microorganisms of the root canals are difficult to eliminate during endodontic treatment. in this study the effect of root canal disinfection with photodynamic therapy (pdt) at different time intervals in comparison to 2.5% sodium hypochlorite (naocl) irrigation and passive ultrasonic irrigation (pui) in extracted teeth colonized with enterococcus faecalis and candida al...

The first sign about evacuating cells has been given to the ancient Egyptian. They evacuate body of dead person (mummy) from its organs. Then whole evacuated water content.

The distinction between viable and dead bacterial cells poses a major challenge in microbial diagnostics. Due to the persistence of DNA in the environment after cells have lost viability, DNA-based quantification methods overestimate the number of viable cells in mixed populations or even lead to false-positive results in the absence of viable cells. On the other hand, RNA-based diagnostic meth...

Orthodontic clear aligner treatment is gaining tremendous popularity. The world market leader Align Technology® and its product Invisalign®. Although numerous patients are treated with Invisalign® aligners, only little known about the cellular effects of material on oral epithelial cells. In present study, we aimed to investigate SmartTrack® directly cultured primary human keratinocytes (HOKs)....