This study reports the development of a specific and sensitive radioimmunoassay and a simple and accurate radial immunodiffusion (RID) assay for the human serum-binding protein for vitamin D and its metabolites (DBP). These immunoassays employed a monospecific antiserum that was prepared in rabbits against human DBP. The radioimmunoassay effectively measured DBP in amounts of 1-10 ng, whereas t...

BACKGROUND Vitamin D status may influence a spectrum of health outcomes, including osteoporosis, arthritis, cardiovascular disease, and cancer. Vitamin D-binding protein (DBP) is the primary carrier of vitamin D in the circulation and regulates the bioavailability of 25-hydroxyvitamin D. Epidemiologic studies have shown direct DBP-risk relations and modification by DBP of vitamin D-disease asso...

Motivation:Todetect and select patternsof transcription factor binding sites (TFBSs) which distinguish genes directly regulated by estrogen receptor-a (ERa), we developed an innovative mixture model-based discriminate analysis for identifying ordered TFBS pairs. Results: Biologically, our proposed new algorithm clearly suggests that TFBSs are not randomly distributed within ERa target promoters...

DBP, a liver-enriched transcriptional activator protein of the leucine zipper protein family, accumulates according to a very strong circadian rhythm (amplitude approx. 1000-fold). In rat parenchymal hepatocytes, the protein is barely detectable during the morning hours. At about 2 p.m., DBP levels begin to rise, reach maximal levels at 8 p.m. and decline sharply during the night. This rhythm i...

PURPOSE To evaluate if diastolic blood pressure (dBP) ≥90 mm Hg during axitinib treatment is a marker of efficacy. EXPERIMENTAL DESIGN The relationship between dBP ≥90 mm Hg and efficacy was retrospectively explored across 5 phase II studies of single-agent axitinib for the treatment of 4 different tumor types. All patients had baseline BP ≤140/90 mm Hg and were stratified into 2 groups based...

dibutyl phthalate (dbp) is a phthalic acid ester and is widely used in polymeric products to make them more flexible. dbp is found in almost every plastic material and is believed to be persistent in the environment. various analytical methods have been used to measure dbp in different matrices. considering the ubiquitous nature of dbp, the most important challenge in dbp analyses is the contam...

The aim of this study was to investigate physical and biological properties of collagen (COL) and demineralized bone powder (DBP) scaffolds for bone tissue engineering. DBP was prepared and divided into three groups, based on various particle sizes: 75-125 µm, 125-250 µm, and 250-500 µm. DBP was homogeneously mixed with type I collagen and three-dimensional scaffolds were constructed, applying ...

Interaction of the Duffy binding protein (DBP) with its erythrocyte receptor is critical for maintaining Plasmodium vivax blood-stage infections, making DBP an appealing vaccine candidate. The cysteine-rich region II is the ligand domain of DBP and a target of vaccine development. Interestingly, most of the allelic diversity observed in DBP is due to the high rate of nonsynonymous polymorphisms...

Two mouse models in which the vitamin D-binding protein (DBP) or megalin, a DBP receptor, were knocked out have recently confirmed that DBP is essential for normal vitamin D homeostasis, and have revealed a major pathway for vitamin D activation (1, 2). DBP is also known as the group-specific component of serum (Gc-globulin). It is a highly polymorphic 58 kDa glycoprotein, which has been used a...

The degradation and adsorption behavior of dibutyl phthalate (DBP) in methanogenic phase refuse was investigated through laboratory microcosm experiments. The results showed that the half-life of DBP in the sterilized refuse was 5.9 times higher than in unsterilized samples, but that it decreased by 35.8% when dominant bacterial strains were added. Different concentrations of DBP did not have o...