A cytochemical study of the lysosomal enzyme beta-glucuronidase in 60 cases of acute leukaemia has shown a qualitative difference in the cytoplasmic distribution of the enzyme between blast cells of the lymphoid and myeloid cell series. This difference provides a useful additional method for cytochemical classification of cell type and is superior in this respect to the other lysosomal enzymes ...

background nephrotic syndrome (ns) characterized by a large amounts of protein into urine and a set of indications that include: protein in urine, low blood protein levels, high cholesterol levels, high triglyceride levels, and swelling. therefore, aim of this study was to investigate the relationship between prognosis of nephrotic syndrome and blood biomarkers level in children of arak city, i...

background attention deficit hyperactivity disorder (adhd) is a multi-factorial disorder that has defined by hyperactivity, impulsivity and attention deficits. various neurotransmitters such as dopamine can play a role in its pathophysiology. the aim of this study was to examine the association of two common single nucleotide polymorphisms in drd2 gene, taq i a (t/c) and taq i b (g/a), with adh...

New techniques have been devised for the cytochemical demonstration of purine nucleoside phosphorylase (PNP) and adenosine deaminase (ADA) activities in unfixed human lymphocytes. A suspension of living lymphocytes is mixed with agarose sol containing the reagents for the detection of PNP or ADA activity on a glass slide. The mixture solidifies. is incubated, and then dried for lightmicroscopic...

The enzymic properties of adenylyl cyclase in purified membrane vesicles from human and boar spermatozoa are described. Plasma membrane vesicles, which appear to be right-side-out, show a marked increase in activity in the presence of the detergent Triton X-100, manganous ion and alkaline pH. Electron-microscope cytochemical assays indicated the presence of adenylyl cyclase in boar and human sp...

Bodies with the morphologic and cytochemical characteristics of peroxisomes have been identified in the satellite and Schwann cells of rat dorsal root ganglia. They are membrane-delimited, round or oval structures which contain a moderately electron-dense matrix but lack a crystalline core. On incubation of the tissue in a cytochemical medium for demonstration of peroxisomes, these bodies show ...

Five canine cutaneous histiocytomas were studied by electron microscopy and esterase cytochemistry. The tumor cells contained irregular nuclei, characteristic lysosomal granules, and perinuclear microfilaments. The cells showed activity with anaphthyl acetate esterase stains. These characteristics are evidence that this tumor originates from the mononuclear phagocyte system. Canine cutaneous hi...