Multidrug resistance to chemotherapy is a major obstacle in the treatment of cancer patients. The best characterised mechanism responsible for multidrug resistance involves the expression of the MDR-1 gene product, P-glycoprotein. However, the resistance process is multifactorial. Studies of multidrug resistance mechanisms have relied on the analysis of cancer cell lines that have been selected...

Ullrich congenital muscular dystrophy is a severe genetically and clinically heterogeneous muscle disorder linked to collagen VI deficiency. The pathogenesis of the disease is unknown. To assess the potential role of mitochondrial dysfunction in the onset of muscle fiber death in this form of dystrophy, we studied biopsies and myoblast cultures obtained from patients with different genetic defe...

Changes in a patient’s patterns of cocaine use are generally considered an important outcome measure of treatment efficacy. Other treatment outcome measures are important as well, but if a treatment does not stop or significantly decrease the intensity of a cocaine addict’s cocaine use, many would question the treatment’s efficacy. Examination of a patient’s urine for evidence of cocaine or coc...

an experiment was conducted to investigate the effect of different types of ovarian cycles on reproductive indices and blood metabolites in 61 holstein cows. blood samples were taken from each cow between day 12 and day 50 postpartum (pp). serum progesterone concentrations were measured to determine the onset of luteal function and different types of luteal dysfunctions in cows. serum glucose, ...

the objective of this study was to determine the effects of diets having different fermentability on blood gases, blood metabolites and ruminal fermentation parameters in holstein dairy cows. four multiparous holstein dairy cows (665 ± 25 kg bw, 170 ± 7.0 dim and 15±1.5 kg of milk yield), fitted with rumen cannulae, were used in a 4×4 latin square design with 28-d periods. corn starch and sucro...

BACKGROUND One adverse side-effect of the immunosuppressive drug cyclosporin A (CsA) is a decrease in glomerular filtration rate (GFR). This effect might be the result of increased glomerular contractions. The present study compared the contractile effects of CsA, cyclosporin G (CsG) and the novel cyclosporin derivative IMM 125 in isolated rat glomeruli and primary cultures of rat mesangial cel...

a study was conducted to evaluate the effect of feeding different levels of protein to black baladi breed kids. weanling baladi kids (n=18; 75 to 90 days old) were selected and individually housed at our experimental farm. kids were divided randomly to one of the three treatments for 12 weeks. the three dietary treatments were: t1: control ration, formulated according to nrc to cover the protei...

a rapid and sensitive hplc method has been developed for the quantification of mirtazapine (mrz), a noradrenergic and specific serotonergic inhibitor antidepressant (nassa) and its two major metabolites n-desmethylmirtazapine (ndm) and 8-hydroxymirtazapine (8-ohm) in human plasma. the separation was achieved using chromolith c18 column and a mobile phase of acetonitrile: phosphate buffer (ph=3,...