The degradation of taurine, isethionate and sulfoacetate in Cupriavidus necator (Ralstonia eutropha) H16 was shown by enzyme assays to be inducible, and each pathway involved sulfoacetaldehyde, which was subject to phosphatolysis by a common sulfoacetaldehyde acetyltransferase (Xsc, H16_B1870) to yield acetyl phosphate and sulfite. The neighbouring genes encoded phosphate acetyltransferase (Pta...

The immunoglobulin G (IgG) binding ZZ domain of protein A from Staphylococcus aureus was fused to the N terminus of the polyhydroxyalkanoate (PHA) synthase from Cupriavidus necator. The fusion protein was confirmed by matrix-assisted laser desorption ionization-time-of-flight mass spectrometry and mediated formation of ZZ domain-displaying PHA granules in recombinant Escherichia coli. The IgG b...

Abstract Polyhydroxyalkanoate or PHA is produced through microbial fermentation of sugar and lipid where in nature it stored as energy carbon source for microorganisms. Most the feedstock used production are derived from biomass they remain cheapest to date. In this study, nipa palm sap was proposed an alternative since underutilized widely distributed along river estuaries Malaysia. This conta...

Cupriavidus necator has a wide metabolic range and naturally creates biopolymer, poly[(R)-3 hydroxybutyrate] (PHB). Using engineering techniques, carbon flux can be directed away from PHB synthesis toward the generation of biofuels bioproducts.Researchers demonstrated production many biofuel products using C. necator, including methyl ketones, isoprenoids terpenes, isobutanol, alkanes alkenes, ...

The accumulation of polyhydroxyalkanoates (PHAs) by microorganisms usually occurs in response to environmental stress conditions. Therefore, it is advantageous choose two-step cultivation. first phase aimed at maximizing biomass production, and only the second phase, after setting suitable conditions, PHA production starts. aim this work was optimize composition minimal propagation medium used ...