A simple drop method for quantitative urine culture was developed and tested in comparison with standard methods for bacterial urinary counts. In a group of 452 urines all yielding Escherichia coli, 74 showed counts of more than 100,000 colonies, and 16 showed counts between 10,000 and 100,000 colonies per ml. Of these 90 urines, 3 of the 16 in the doubtful group were false negative with the dr...

A modified slide culture method which combines advantages found in several slide culture methods is described. A block of inoculated nutrient agar sandwiched between two sterile cover glasses is placed in a plastic petri dish containing water agar. After adequate growth has occurred, the slide culture is disassembled and mounted in a conventional manner.

edigraphic.com Blastocystis hominis is a strict anaerobic protozoan that occurs in the intestine of humans and other mammals (Zierdt, 1991). Recently, phylogenetic analyses of ribosomal RNAs place B. hominis within the straminopiles (Silberman et al., 1996). Its pathogenic role in human beings is still disputed by some investigators (Markell and Udkow, 1986; Miller, 1988). The mechanism by whic...

Background  Isolation of adipose tissue mesenchymal stem cells (MSCs) with current enzymatic methods has some limitations. For example, it is costly and time-consuming and results in a heterogeneous cell population that making compromise proliferation and differentiation of MSCs. Also, it is accompanied with the increased risk of culture contaminations because of several handling steps.In this...