Acetylcholinesterase (AChE) is an essential enzyme that terminates cholinergic transmission by a rapid hydrolysis of the neurotransmitter acetylcholine. AChE is an important target for treatment of various cholinergic deficiencies, including Alzheimer's disease and myasthenia gravis. In a previous high throughput screening campaign, we identified the dye crystal violet (CV) as an inhibitor of A...

Several studies have validated the effectiveness of narrow-band imaging (NBI) in estimating invasion depth of early colorectal cancers. However, comparative diagnostic accuracy between NBI and chromoendoscopy remains unclear. Other than crystal violet, use of acetic acid as a new staining method to diagnose deep submucosal invasive (SM-d) carcinomas has not been extensively evaluated. We aimed ...

Bacterial colonization of polymeric medical aids causing so-called nosocomial infections is one of the main problems linked with the extensive use of polymeric devices in clinical applications. In this study, the properties of medical-grade polyvinyl chloride were modified by incorporating crystal violet into its bulk structure. Sodium montmorillonite was used as a carrier for the crystal viole...

The effect of acid treatment on the adsorptive capacity a Brazilian palygorskite to remove crystal violet (CV) and congo red (CR) dyes was investigated. raw acid-treated by different HCl solutions (2, 4, 6 mol/L). modifications structure were investigated using X-ray diffraction, fluorescence, Fourier-transform infrared spectroscopy, N2 adsorption/desorption, thermogravimetric differential ther...

A series of aminotriarylmethane dyes were examined for binding to the nicotinic acetylcholine receptor (AChR) from Torpedo californica. Several compounds were found to bind to the noncompetitive antagonist site of the AChR as demonstrated by inhibition of [3H]phencyclidine binding; apparent KD values ranged from 50 nM to >100 microM. One dye with high affinity, crystal violet, revealed a greate...

Borzani and Vairo (1959) reported that grampositive and gram-negative microorganisms are fundamentally different with respect to crystal violet adsorption. They claimed that the binding of crystal violet by gram-positive cells followed the Freundlich and the Langmuir adsorption laws; whereas the binding of crystal violet by gram-negative cells did not follow any known adsorption law. The presen...

Organic colourants have important applications in many fields. Their photostability is an characteristic. Several methods to study photodegradation were compared this work. Eosin Y (C.I. Generic name: Acid Red 87, EY) and crystal violet Name: Basic Violet 3, CV) used as test compounds, both solution dyed on silk. Commonly applied included, viz. Xenotest, Microfading-Tester, light-box (Spectroli...

Silver nanoparticles with a tuneable λ max were produced as colloids by heterogeneous nucleation. The synthesis process is both fast and repeatable, producing stable PVA capped nanoparticles. The colloid's effectiveness in the SERRS system was investigated using Rhodamine 6G, R6G, Crystal Violet, CV, and Malachite Green, MG, as probe molecules. A clear sensing trend was observed, where the Rama...

Crystal violet exhibited characteristics of an uncoupler of oxidative phosphorylation, i.e. it released respiratory control, hindered ATP synthesis, enhanced ATPase activity, and produced swelling of isolated rat liver mitochondria. Maximal stimulation of respiration, ATPase activity, and swelling was observed at a concentration of 40 microM. The inhibition of State 3 respiration by oligomycin ...

Adsorption of cationic dye crystal violet (CV) onto a modified walnut shell (WS) adsorbent was investigated. Combined treatment WS using urea and sodium dodecylsulfate (SDS) carried out. Surface modification adsorbents confirmed by FTIR analysis, pHpzc measurements, elemental SEM-EDX analysis. In order to optimize the adsorption conditions, effect solution pH, dose CV concentration studied mean...