The method of vitrification has been widely used for cryopreservation. However, the effectiveness of this method for mammalian oocytes could be improved by optimizing each step of the process. In the present study, we tested the effects of varying several key parameters to determine the most effective protocol for mouse oocyte vitrification. We found that cryoprotectant containing ethylene glyc...

AIM Oocyte cryopreservation remains largely experimental, with live birth rates of only 2-4% per thawed oocyte. In this study, we present a nanoliter droplet technology for oocyte vitrification. MATERIALS & METHODS An ejector-based droplet vitrification system was designed to continuously cryopreserve oocytes in nanoliter droplets. Oocyte survival rates, morphologies and parthenogenetic devel...

Objective: Evaluate the use of Ethylene Glycol (EG), Dimethyl Sulfoxide (DMSO), Sucrose and Fetal Bovine Serum (FBS) as cryoprotectants their effect on organization chromosomes arrangement microtubules, during vitrification process in goat oocytes matured vitro development preimplantation embryos produced vitro.
 Design/methodology/approach: In were divided into 3 groups (control group, cr...

Gamete cryopreservation combined with in vitro embryo production (IVP) and cryopreservation of resultant embryos are important technologies for gene banking in domestic animals. Especially, cryopreservation of embryos and oocytes offers the possibility for the preservation of female genetic lines. Although several vitrification protocols have been developed for the cryopreservation of embryos a...

This study was conducted to compare the efficiency of four vitrification methods: straws in goblet, straws in Hasler device, cryloop and cryotop methods on blastocyst viability and the extent of apoptosis using the TUNEL technique in bovine embryos. Blastocysts were produced in vitro by standard procedures. The base medium for vitrification was Syngro holding medium. Embryos were vitrified by t...

OBJECTIVE The aim of this study was to compare vitrification optimization of mouse embryos using electron microscopy (EM) grid, cryotop, and thin plastic strip (TPS) containers by evaluating developmental competence and apoptosis rates. METHODS Mouse embryos were obtained from superovulated mice. Mouse cleavage-stage, expanded, hatching-stage, and hatched-stage embryos were cryopreserved in E...

Cryopreservation of oocytes would serve as an alternative to overcome the limited availability of dromedary camel oocytes and facilitate improvements in IVP techniques in this species. Our goal was to develop a protocol for the vitrification of camel oocytes at the germinal vesicle (GV) stage using different cryoprotectant combinations: 20% EG and 20% DMSO (VS1), 25% EG plus 25% DMSO (VS2) or 2...

Careful genetic management, including cryopreservation of genetic material, is central to conservation of the endangered Mexican gray wolf. We tested a technique, previously used to vitrify human and domestic animal oocytes, on oocytes from domestic dogs as a model and from the endangered Mexican wolf. This method provided a way to conserve oocytes from genetically valuable older female Mexican...