Background An evaluation of the developmental competence of vitrified mouse germinal vesicle (GV) oocytes with various equilibration and vitrification times; in the presence or absence of cumulus cells and by comparison between the cryotop method and straws was performed. MaterialsAndMethods Mouse GV oocytes were considered in cumulus-denuded oocytes (CDOs) and cumulus-oocyte complexes (COCs) g...

OBJECTIVE(S) The examination of the possibility of applying lower CPA- concentrations and obtaining the similar results to those using higher concentrations; as it is shown, the toxicity of the CPAs used in vitrification approach will diminish. MATERIALS AND METHODS Following vitrification/warming, oocytes were subjected to PZD/ICSI. SRs, FRs, and DRs were recorded. SRs and DRs of the embryos...

Background: The aim of the study was to compare the effects of two different concentrations of cryoprotectants by Cryotop vitrification on survival and Heat shock protein 72 (Hspa1a) expression of two-cell mouse embryos. Materials and Methods: Different cryoprotectants’ concentrations of the combination of dimethyl sulfoxide (DMSO) and ethylene glycol (EG) were used and compared with each other...