Cryo-electron tomography (cryo-ET) allows researchers to image cells in their native, hydrated state at the highest resolution currently possible. The technique has several limitations, however, that make analyzing data it generates time-intensive and difficult. Hand segmenting a single tomogram can take from hours days, but microscope easily generate 50 or more tomograms day. Current deep lear...

Cryo-electron microscopy（cryo-EM）is a powerful method to visualize high resolution structures of biomolecules under physiological condition. Since cryo-EM images represent conformational ensemble the target biomolecule, is applicable analyze biomolecular dynamics such as metastable conformations and/or free-energy landscape in changes. Here, we briefly introduce our deduce from and compare it w...

We provide a catalog of 3D cryo soft X-ray tomography (cryo-SXT) images obtained from ∼6 to 12μm thick mouse adenocarcinoma cells. Included are multiple representative images of nuclei, nucleoli, nuclear membrane, nuclear membrane channels, mitochondria, lysosomes, endoplasmic reticulum, filaments and plasma membrane, plus three structures not previously described by cryo-SXT, namely Golgi, mic...

Cryo-electron microscopy (cryo-EM) plays an increasingly more important role in structural biology. With the construction of an arm of the Chinese National Protein Science Facility at Tsinghua University, biological cryo-EM has entered a phase of rapid development in China. This article briefly reviews the history of biological cryo-EM in China, describes its current status, comments on its imp...

INTRODUCTION Cryoablation is used increasingly for the treatment of atrioventricular nodal reentrant tachycardia (AVNRT). We sought to compare the long-term outcomes of cryoablation (Cryo) vs. radiofrequency (RF) ablation for the treatment of AVNRT in children. METHODS Two groups of consecutive patients were analyzed retrospectively: the RF group, 20 patients (60% males, mean age 13.25 +/- 2....

Recent technical advances have revolutionized the field of cryo-electron microscopy (cryo-EM). However, most monomeric proteins remain too small (<100 kDa) for cryo-EM analysis. To overcome this limitation, we explored a strategy whereby a monomeric target protein is genetically fused to a homo-oligomeric scaffold protein and the junction optimized to allow the target to adopt the scaffold symm...

We present the development of a microfluidically cryo-cooled planar coil for magnetic resonance (MR) microscopy. Cryogenically cooling radiofrequency (RF) coils for magnetic resonance imaging (MRI) can improve the signal to noise ratio (SNR) of the experiment. Conventional cryostats typically use a vacuum gap to keep samples to be imaged, especially biological samples, at or near room temperatu...

BACKGROUND Transbronchial Cryobiopsy (Cryo-TBB) represents a new approach for sampling lung tissue in interstitial lung disease (ILD). OBJECTIVES Comparing the reliability of Cryo-TBB results and the procedure's complication rates with the procedure of surgical lung biopsy (SLB). METHODS ILD subjects with the need of lung tissue sampling are analyzed in this prospective trial. To determine ...

Cryogenic electron microscopy (cryo-EM) is a powerful tool for imaging liquid and semiliquid systems. While cryogenic transmission electron microscopy (cryo-TEM) is a standard technique in many fields, cryogenic scanning electron microscopy (cryo-SEM) is still not that widely used and is far less developed. The vast majority of systems under investigation by cryo-EM involve either water or orga...