Changes in trigonelline content and in biosynthetic activity were determined in the cotyledons and embryonic axes of etiolated mungbean (Phaseolus aureus) seedlings during germination. Accumulation of trigonelline (c. 240 nmol per pair of cotyledons) was observed in the cotyledons of dry seeds; trigonelline content decreased 2 d after imbibition. Trigonelline content in the embryonic axes incre...

Phytohemagglutinin (PHA), the major seed lectin of the common bean (Phaseolus vulgaris), is found largely in the cotyledons, but is also present in the embryonic axis. At mid-maturation, the percentage of total protein synthesis which is directed towards making PHA is 5 to 10 times greater in the cotyledons than in the axes. This lower rate of synthesis in the axes is correlated with a lower ab...

During germination and early growth of the castor bean (Ricinus communis) nitrogenous constituents from the endosperm are transferred via the cotyledons to the growing embryo. Exudate collected from the cut hypocotyl of 4-day seedlings contained 120 millimolar soluble amino nitrogen and glutamine was the predominant amino acid present, comprising 35 to 40% of the total amino nitrogen. To determ...

Fusicoccin inhibits the germination of pea (Pisum sativum L. cv Progress 9) seeds by decreasing the growth of the embryonal axis and by stimulating the fresh weight increase of the cotyledons. The growth of isolated embryonal axes in the presence of sucrose and KCl is stimulated by fusicoccin. The effect of fusicoccin on the seeds is not counteracted by sucrose and KCl. Fusicoccin promotes pref...

Treatment of excised marrow (Cucurbita pepo L., zucchini) cotyledons in darkness with 45 μM BA caused a marked stimulation of the growth accompanied by a strong decrease in cotyledons relative dry weight. PMSF – a well known inhibitor of both thioland serine-type proteases, present in the aqueous solutions either alone or with the cytokinin, inhibited significantly the growth of cotyledons and ...

sugar present in the endosperm was predominantly, and in the cotyledon almost exclusively, sucrose. Anatomical features were described which contribute to the efficiency of the cotyledons as organs of absorption and transmittal of sucrose to the embryonic axis, where hexoses are much more prevalent. The ability of the cotyledons to absorb sucrose survived removal of the endosperm from the seedl...

In contrast to earlier reported results of similar experiments in peas, in which almost no increase in protease activity occurred in incubated detached cotyledons, we report here an increase in protease activity in both attached and detached bean cotyledons. Detached bean cotyledons showed continually increasing protease activity up to the 12th day, while that in attached cotyledons declined af...

The effect of Ca on senescence was followed in detached cucumber (Cucumis sativus L.) cotyledons floating on various solutions in the dark. Compared with those in water, cotyledons in 10(-4) molar CaCl(2) exhibited reduced chlorophyll loss and H(2)O(2) production, reduced and delayed ethylene production, and did not undergo a burst in CO(2) production. In contrast, Mg had little effect on cotyl...

Succinate dehydrogenase (succinate: phenazine methosulfate oxidoreductase, EC 13.99.1) activity in crude mitochondrial fraction from pea (var. Alaska) cotyledons increased during seed imbibition to reach a maximum after about 12 hours. The increase was not inhibited by either cycloheximide or D(-)dweo-chloramphenicol. The postmicrosomal fraction from dry cotyledons, but not that from fully imbi...

Polyadenylated RNA was isolated from total RNA extracted from cotyledons of ungerminated or 18-hour-germinated chick-pea seeds by affinity chromatography on oligo(dT)-cellulose. Both poly(A)-containing RNA fractions exhibited a template activity when assayed in two cell-free translation systems, wheat germ extracts, and nuclease-treated reticulocyte lysates. Translation of preformed mRNA from c...