Objective To determine the toxicity of the potential fungicide chlorhexidine gluconate (as a pure 0.2% solution and as a commercially available skin antiseptic diluted to contain 0.2% chlorhexidine gluconate) to the cornea and adjacent ocular structures. Methods An experimental study was performed at the Animal Facility of the Massachusetts Eye and Ear Infirmary. Pure chlorhexidine gluconate 0....

PURPOSE To determine the efficacy of photodynamic therapy (PDT) with verteporfin (Visudyne; Novartis AG, Basel, Switzerland) for treatment of corneal neovascularization in a rabbit eye model. METHODS Corneal neovascularization was induced in Dutch belted rabbits by placing an intrastromal silk suture near the limbus. Verteporfin was administered by intravenous injection at a dose of 1.5 mg/kg...

PURPOSE Herpes keratitis (HK) is the leading cause of cornea-derived and infection-associated blindness in the developed world. Despite the availability of effective antivirals, some patients develop refractory disease, drug-resistant infection, and topical toxicity. A nonpharmaceutical treatment modality may offer a unique advantage in the management of such cases. This study investigated the ...

PURPOSE Nearly all eye drops contain preservatives to decrease contamination. Nonpreservatives such as disodium-ethylene diamine tetra-acetate (EDTA) and phosphate-buffered saline are also regularly added as buffering agents. These components can add to the toxicity of eye drops and cause ocular surface disease. To evaluate the potential toxicity of these common components and their comparative...

Aiming at the replacement of animal experiments in eye irritation testing, we have established a multilay ered cornea model comprising the co-culture of all three corneal cell types. It was the objective of this study to optimise serum-free culture conditions to preserve both growth and phenotype of an SV40-immortalised human corneal keratocyte cell line (HCK). Our results revealed that HCK con...

PURPOSE The authors conducted in vivo assessment of corneal endothelial toxicity of air and SF6 in the feline model. This research was motivated by the increased use of air in anterior segment surgery in human subjects. METHODS This was a prospective masked study. The eyes of 16 healthy adult cats were randomly assigned for the injection of 0.7 mL air into the anterior chamber of one eye and ...

PURPOSE To evaluate corneal endothelium alterations after applying mitomycin C to the sclera using transmission and scanning electron microscopy, correlating alterations with time, concentration, and evaluation methods. METHODS The corneal endothelium of both eyes of 32 albino rabbits was evaluated and distributed into four groups of 8. Mitomycin C was applied under a scleral flap in the righ...

normal 0 false false false en-us x-none ar-sa background : the aim of this study was to determine the correlation between corneal hysteresis (ch) and the corneal resistance factor (crf), which are both novel methods of analyzing ocular rigidity/elasticity, and various corneal characteristics, mainly corneal volume in normal subjects. methods : this cross-sectional study included 500 normal eyes...

T he Draize rabbit eye irritation test developed in the 1940’s is even today the only eye toxicity test officially accepted in the OECD countries for regulatory purposes in the classification of slightly and moderately irritating chemicals. It is based on the subjective scoring of three tissues of the eye: the cornea, the conjunctiva and the iris. The Draize test has been widely criticized for ...

Comment 5-FU is an antimetabolite which interferes with normal cell mitosis. It interfers with the S phase and the G2 phase of the cell cycle which correspond, respectively, with the synthesis of DNA and cellular components required for mitosis.' As corneal epithelial cells are constantly undergoing replication they are particularly susceptible to its toxic effects. Reduction of dosage has been...