Objective: this study aims to develop a methodology for detecting mutations in the K13 gene and determine mutation frequencies clinical samples. Method: total DNAs were extracted blood samples collected from 50 patients failure with artemisinin treatmentin Central Highlands region. A fragment of was amplified, purified, sequenced by Sanger method. The sequences analysed using Bioedit aligned re...

Background: P53 tumor suppressor gene mutation is one of the most common genetic alterations in human malignancies. The mutated from of the gene is stable and can be detected with immunohistochemistry methods. There is much controversy about the expression rate of this gene in malignant melanoma. Objective: To determine the frequency of the P53 antigen expression by sex, age, type and thickness...

Purpose: The aim of this study is to explore the effects gossypin on matrix metalloproteinases -2 (MMP-2) and MMP-9 genes in prostate cancer cells. Materials Methods: PC3 cells were grown culture treated with three different concentrations (25-50-100 µg/ml) cisplatin (50 µM) as a positive control. First, RNA isolation was performed. Then, cDNA synthesis performed RT-PCR used evaluate mRNA expre...

0

49 HYDROCORTISONE IMPROVED HAEMODYNAMICS AND FLUID REQUIREMENT IN SURVIVING BUT NOT NONSURVIVING OF SEVERELY BURNED PATIENTS The administration of hydrocortisone can lead to a reduction of catecholamines and improved outcome in septic patients. However, no data exist regarding the use of hydrocortisone in burn patients although, in such patients, the reduction of vasopressors may be even more c...