The searching of human housekeeping (HK) genes has been a long quest since the emergence of transcriptomics, and is instrumental for us to understand the structure of genome and the fundamentals of biological processes. The resolved genes are frequently used in evolution studies and as normalization standards in quantitative gene-expression analysis. Within the past 20 years, more than a dozen ...

coach's emotional intelligence inspires health and development of sport teams. balancing emotion and thought, emotional intelligence influences conflict conditions in workplace environment. the aim of this study was to explore the relationship between emotional intelligence and conflict management styles in the active karate coaches in isfahan province in 2012. 285 subjects were selected f...

Analysis of RNA expression using techniques like real-time PCR has traditionally used reference or housekeeping genes to control for error between samples. This practice is being questioned as it becomes increasingly clear that some housekeeping genes may vary considerably in certain biological samples. We used real-time reverse transcription PCR (RT-PCR) to assess the levels of 13 housekeeping...

5 Normalization by means of reference/housekeeping genes 14 5.1 ∆Cq method using a single housekeeper . . . . . . . . . . . . . . . . . . . . 14 5.2 ∆Cq method using a combination of housekeeping genes . . . . . . . . . . . 15 5.3 2−∆∆Cq method using a single housekeeper . . . . . . . . . . . . . . . . . . 16 5.4 2∆∆Cq method using a combination of housekeeping genes . . . . . . . . . . 19 5.5 ...

BACKGROUND Contaminated environmental surfaces may play an important role in transmission of some healthcare-associated pathogens. In this study, we assessed the adequacy of cleaning practices in rooms of patients with Clostridium difficile-associated diarrhea (CDAD) and vancomycin-resistant Enterococcus (VRE) colonization or infection and examined whether an intervention would result in improv...

There is a need for easy, practical, reliable and robust techniques for the identification and classification of bacterial isolates to the species level as alternatives to 16S rRNA gene sequence analysis and DNA-DNA hybridization. Here, we demonstrate that multilocus sequence analysis (MLSA) of housekeeping genes is a valuable alternative technique. An MLSA study of 10 housekeeping genes (atpD,...

restoring fucosylation. In the absence of fucose, the FX / mice display a profound neutrophilia. Part of this neutrophilia can be explained by loss of selectin ligands (Sialyl Lewis x contains fucose), but proliferation of myeloid progenitor cells suggests that myelopoiesis is being stimulated. Zhou and coworkers have now examined the proliferation of myeloid lineages in FX / mice and attribute...

the armenian-turkish conflict except the disagreement between the two countries has also developed in the regional and international levels. it is becoming the conflict of interests and competition of superpowers in regional and international sphere, which has its great influence on the complication of the process of the conflict between the two countries. taking into consideration the disagree...