this survey was conducted on 133 egge that they were collected from isphahan markets, eggs are tested for aerobic mesophill bacteria (total count) and 186 eggs were tested for salmonella spp. contamination. the results showed 3000 to 10 million colony per gram bacteria counted in egg samples. the mean colony count was 1693000.7 percentage samples rich to mean colony count. (95% accuracy,sd2.232...

Honey bees are important pollinators of agricultural crops. Pathogens and other factors have been implicated in high annual losses of honey bee colonies in North America and some European countries. To further investigate the relationship between multiple factors, including pathogen prevalence and abundance and colony health, we monitored commercially managed migratory honey bee colonies involv...

Gonococci containing a 24 X 10(6)-dalton conjugal plasmid were able to mobilize for transfer a smaller, non-self-transmissible penicillinase (Pcr) plasmid with high frequency under appropriate conditions. In some strains, over 10% of donor colony-forming units transferred the Pcr plasmid in a mating of less than 2 h, which suggests that the conjugal system was naturally derepressed. Colony-opac...

AIMS To develop a real-time (rt) PCR for species differentiation of thermophilic Campylobacter and to develop a method for assessing co-colonization of pigs by Campylobacter spp. METHODS AND RESULTS The specificity of a developed 5' nuclease rt-PCR for species-specific identification of Campylobacter jejuni, Campylobacter coli, Campylobacter lari, Campylobacter upsaliensis and of a hipO gene ...

Site-directed mutagenesis: colony growth This month's question from the Molecular Biology Forums (online at molecularbiology. forums.biotechniques.com) comes from the " General Methods " section. Entries have been edited for concision and clarity. Mentions of specific products and manufacturers have been retained from the original posts, but do not represent endorsements by, or the opinions of,...

Isolates showing different and similar colony morphologies were selected from spread plates of bacteria from seawater samples taken in the northern Adriatic Sea. All isolates were characterised by restriction fragment length polymorphism (RFLP) patterns of their PCR-amplified 16S rRNA gene and by 95 physiological tests (Biolog system). Cluster analysis of both genetic and phenotypic patterns sh...