different grapevine cultures that were inoculated with Plasmopara viticola as a host pathogen and Pseudoperonospora cubensis as a non-host pathogen. The results of the expression analysis showed that the PR1 mRNA level in Vitis vinifera cv. Riesling and Vitis riparia cv. Gloire de Montpellier is mainly affected by the culture system. PR1 is constitutively expressed in callus cultures. In vitro ...

Lignin was used as a natural adhesive to manufacture Vitis vinifera fiberboards. The fiberboards were produced at laboratory scale by adding powdered lignin to material that had previously been steamexploded under optimized pretreatment and pressing conditions. The kraft lignin used was washed several times with an acidic solution to eliminate any contaminants and low molecular weight compounds...

Phytochemical constituents of Vitis wenchowensis were investigated for the first time. A new resveratrol trimer, wenchowenol (1), was isolated from the roots and stems of Vitis wenchowensis along with four known stilbenoids 2-5. The structure and relative configuration of 1 were established on the basis of spectral evidence, especially HMBC and NOESY experiments. It showed potent antioxidant ac...

Next-generation sequencing technologies promise to dramatically accelerate the use of genetic information for crop improvement by facilitating the genetic mapping of agriculturally important phenotypes. The first step in optimizing the design of genetic mapping studies involves large-scale polymorphism discovery and a subsequent genome-wide assessment of the population structure and pattern of ...

The polyphenolic composition and antioxidant properties of the methanol leaf extract of Vitis × labruscana cv. ‘Isabella’ has been performed by means of different spectrophotometric antioxidant methods. Intracellular ROS production was measured by cell fluorescence after loading with 2',7'-H2DCF-DA. The extract promising efficacy has laid the basis to its phytochemical characterization. Three h...

SSR (Simple Sequence Repeat) markers and SRAP (Sequence Related Amplified Polymorphism) markers were used to construct a genetic linkage map of Vitis amurensis Rupr. The mapping population consists of 94 individuals derived from the selfing of Beibinghong, a V. amurensis cultivar. The map covers a total length of 1123cM on 21 linkage groups with an average distance between adjacent markers of 4...

Grafting vines as a means of propagation was known as early as the 2 century B.C. (Cato in his treatise De agri cultua.). The use of rootstocks in Vitis, however, was not extensively used until 1880 because it was the only effective method to combat the devastating root louse phylloxera, Phylloxera vitifoliae (Fitch), (Coombe, 1999). In California, grape growers have been using rootstocks for o...

Agrobacterium vitis, the causal agent of grape crown gall, can have severe economic effects on grape production. The bacterium survives systemically in vines and, therefore, is disseminated in propagation material. We developed an assay for use in indexing programs that is efficient and sensitive for detecting A. vitis in grape tissue. Initially, real-time polymerase chain reaction (PCR) primer...

Resveratrol is a major phytoalexin produced by plants in response to various stresses and promotes disease resistance. The resistance of North American grapevine Vitis rupestris is correlated with a hypersensitive reaction (HR), while susceptible European Vitis vinifera cv. 'Pinot Noir' does not exhibit HR, but expresses basal defence. We have shown previously that in cell lines derived from th...

Factors influencing Agrobacterium-mediated transformation of 15 Vitis rotundifolia, Vitis rupestris, Vitis vinifera and Vitis hybrids were explored. A green fluorescent protein/neomycin phosphotransferase II (GFP/NPT II) fusion gene was used to measure transient and stable transgene expression levels. Embryogenic cell cultures at different developmental stages were co-cultivated with Agrobacter...