AIM This study evaluated the influence of acid-etching time on collagen exposure in adhesive interfaces established on primary and permanent dentin. MATERIALS AND METHODS Flat dentin surfaces were produced on sound primary molars and premolars (n = 8). The surfaces were divided into mesial and distal halves, and each half was etched with phosphoric acid for 5 or 15 seconds. The teeth were ran...

A Large scale of full-thickness skin defects is lack of auto-grafts and which requires the engineered skin substitutes for repair and regeneration. One major obstacle in skin tissue engineering is to expand epidermal stem cells (ESCs) and develop functional substitutes. The other one is the scaffold of the ESCs. Here, we applied type I collagen-modified chitin membrane to form collagen-chitin b...

Skin aging is influenced by two distinct processes: intrinsic and extrinsic aging. The latter regroups the external factors influencing skin aging, including cigarette smoke sun rays. While these factors, studied separately, are known to cause premature effects of their synergy have been poorly characterized. objective this project assess harmful impact on Healthy substitutes were produced acco...

BACKGROUND There is a paucity of scientific information on the histologic changes associated with the clinical response to microdermabrasion. OBJECTIVE In this study we sought to evaluate those changes in the skin after microdermabrasion, using a porcine model. METHODS Using the left flank of an 8-week-old pig, we tattooed 4 areas with "A," "B," "C," and "D." Each of these areas was subdivi...

Background: Extensive full-thickness burns require replacement of both epidermis and dermis. In designing skin replacements, the goal has been to re-create this model and make a product which has both essential components. Methods: In the present study, we developed procedures for establishing confluent, stratified layers of cultured human keratinocytes on the surface of modified collagen-chito...

background: extensive full-thickness burns require replacement of both epidermis and dermis. in designing skin replacements, the goal has been to re-create this model and make a product which has both essential components. methods: in the present study, we developed procedures for establishing confluent, stratified layers of cultured human keratinocytes on the surface of modified collagen-chito...

The organization of collagen fibrils in the human cornea and sclera was studied by scanning electron microscopy, after digestion of cellular elements by sodium hydroxide, and by conventional transmission electron microscopy. The collagen fibrils in the cornea had a uniform diameter of about 25 nm. In Bowman's layer, individual collagen fibrils were interwoven densely to form a felt-like sheet. ...

Objective- To determine the effect of collagen type I on hydroxyproline content in Achilles tendon in dogs. Design- Experimental in vivo study. Animals- A total of 12 adult dogs. Procedure- Under general anesthesia and aseptic condition the dorsal surface of right Achilles tendon was exposed and after complete separation of  connective tissue, it was splitted (striking 10 times) in full thic...