Excision of all leaves and buds of Coleus blumei Benth. plants reduced xylem cell and sieve tube regeneration a highly significant amount around a wound in internode number 5 when compared with regeneration in intact (wounded) plants. Application of indoleacetic acid (IAA-(14)C) to the cut surface of internode number 2 restores regenerative activity around the wound in internode number 5. Radio...

Cell suspension cultures o f Coleus blumei Benth. producing high amounts o f rosmarinic acid were used to study the biosynthetic pathway o f this caffeic acid ester. One o f the involved en­ zymes, the hydroxyphenylpyruvate reductase (HPPR), is characterized in this paper. HPPR catalyzes the NAD(P)H dependent reduction o f /^-hydroxyphenylpyruvate to /»-hydroxyphenyllactate. The enzyme develope...

An investigation of the effects of ethylene pretreatment on several facets of auxin metabolism in Coleus blumei Benth "Scarlet Rainbow" revealed a number of changes presumably induced by the gas. Transport of indoleacetic acid-1-(14)C in excised segments of the uppermost internode was inhibited by about 50%. Decarboxylation of indoleacetic acid-1-(14)C by enzyme breis was not affected by the pr...

The complete nucleotide (nt) sequence of the original coleus yellow viroid (CYVd) from Solenostemon scutellarioides, 'Golden Bedder', has been determined. The covalently closed single-stranded CYVd RNA molecule consists of 248 nt residues which assumes a rod-like secondary structure when folded in the model of lowest free energy. The sequence was determined by direct sequencing of RNA and from ...

Transport and metabolism of IAA-1-(14)C in Coleus blumei Benth. was studied by means of a combination of liquid scintillation counting, autoradiography and thin-layer chromatography. Transport of IAA in petiole segments of increasing age (No. 2-8) was strictly polar in a basipetal direction. No acropetal movement occurred in either young or old tissues. The greatest amount, expressed as a perce...