The in vitro effects of thrombomodulin on the inactivation of single chain urokinase-type plasminogen activator (scu-PA) by thrombin were investigated by incubating scu-PA with varying concentrations of human thrombin, in both the absence and presence of soluble rabbit thrombomodulin. 50% inactivation of scu-PA occurred in 45 min at 160 ng/ml thrombin in the absence of thrombomodulin and at 4.6...

The present study was undertaken to evaluate the effect of soil amendment with urea coated with nimin (neembased product with neem-triterpenes) and oils of neem, castor, and rocketsalad on the root-knot nematode, Meloidogyne incognita, and plant growth of mung in pots. Soil amendment with urea coated with different doses viz., 0.02g, 0.04g and 0.06g/pot of nimin and oils of neem, castor rocket-...

A recombinant chimeric plasminogen activator (GHRP-scu-PA-32K), consisting of the tetrapeptide Gly-His-Arg-Pro fused to the N-terminus of the low-molecular single-chain urokinase-type plasminogen activator (Leu144^Leu411), was produced by expression in CHO cells. The stable expression cell line was selected for large-scale expression. The product was purified by antibody^Sepharose affinity chro...

Synonymous codon usage (SCU) varies widely among human genes. In particular, genes involved in different functional categories display a distinct codon usage, which was interpreted as evidence that SCU is adaptively constrained to optimize translation efficiency in distinct cellular states. We demonstrate here that SCU is not driven by constraints on tRNA abundance, but by large-scale variation...

Single-chain urokinase-type plasminogen activator (scu-PA) possesses enzymatic activity that increases by 2-3 orders of magnitude upon binding to its cellular cofactor, the u-PA receptor (u-PAR), hence activating an enzymatic cascade initially composed of zymogens. The present study demonstrates that plasminogen activator inhibitor type 3 (PAI-3) reversibly inhibits scu-PA in solution, maintain...

Single-chain urokinase plasminogen activator (scu-PA) that had been modified with N-succinimidyl-3-(2-pyridyldithio)propionate was covalently linked by disulfide bonds to the Fab' of a monoclonal antibody specific for the beta-chain of fibrin (antibody 59D8). scu-PA-59D8 Fab' conjugate was separated from free scu-PA and two-chain urokinase coupled to 59D8 Fab' by two-step affinity chromatograph...

Abstract The characteristics of peat soil with high organic and moisture content, also compressibility can cause structural failure due to excessive settlement. Improvement settlement parameters be conducted using calcite precipitation method soybean as bio-catalyst, namely Soybean Crude Urease-Calcite Precipitation (SCU-CP). SCU-CP is one the grouting methods improve consistency so that bearin...

The purpose of this study was to prepare, optimize, and characterize a cationic lipid nanoparticle (CLN) system containing multicomponent drugs using a molecular dynamics model as a novel method of evaluating formulations. Puerarin (PUE) and scutellarin (SCU) were used as model drugs. CLNs were successfully prepared using melt-emulsion ultrasonication and low temperature-solidification techniqu...

BACKGROUND An enriched environment for residents with dementia may have a positive effect on the rest-activity rhythm. A small scaled homelike special care unit might be such an enriched environment. The present study shows whether the rest-activity rhythm of residents with moderate to severe dementia responds positively to a transfer from a regular Special Care Unit (SCU) to a small scaled hom...

Urea coating has been conducted using polylactic acid (PLA) blended with an industrial softwood kraft lignin. The latter was pre-functionalized via esterification or Mannich reaction. The coated urea becomes therefore a highly effective nitrogen fertilizer due to the controlled release after the coating, the slow release from the organically bound nitrogen structure formed after the Mannich rea...