Lactuca sativa is widely used as a leafy vegetable belonging to the Asteraceae family. Cotyledonary leaves of in vitro lettuce seedlings were transformed by plasmids containing the GCHI gene. Also the effects of number of days of pre-cultivation and co-cultivation of explants were examined on the transformation frequency. After co-cultivation, the explants were transferred onto the regeneration...

Switchgrass is one of the most important biomass/bioenergy crops. For its improvement as a feedstock through biotechnological approach, we have developed a high throughput Agrobacterium-mediated transformation system for cv. Alamo and two new elite cultivars, Performer and Colony. Highly regenerable and transformation-competent embryogenic calli were identified and used for genetic transformati...

The study was conducted to standardize a protocol for Agrobacterium-mediated genetic transformation of buffel grass (Cenchrus ciliaris L.). Embryogenic calli, produced from one-year-old mature seeds of buffel grass, were used as target cells for Agrobacterium-mediated transformation. A. tumefaciens strain LBA4404, harbouring pCAMBIA-1301 or pCAMBIA-2301, was used for co-cultivation with embryog...

Stocks of Rous sarcoma virus Bryan strain were mutagenized using a bromodeoxyuridine treatment immediately after infection. Thirty temperature-sensitive (ts) mutants defective in transformation (td) were isolated by a replica plating technique. Twenty of these mutants were preliminarily characterized and found to be defective in late functions related to transformation. These mutants were used ...

A spiking neural network (SNN) model trained with spiking-timingdependent-plasticity (STDP) is proposed to perform a 2D co-ordinate transformation of the polar representation of an arm position to a Cartesian representation in order to create a virtual image map of a haptic input. The position of the haptic input is used to train the SNN using STDP such that after learning the SNN can perform t...

The results presented in this work support the hypothesis that Agrobacterium-mediated transformation of sorghum is feasible, analogous to what has been demonstrated for other cereals such as rice, maize, barley and wheat. The four factors that we found most influenced transformation were: the sensitivity of immature sorghum embryos to Agrobacterium infection, the growth conditions of the donor ...

Metamodel evolution and model co-evolution are considered to be essential ingredients for the successful adoption of model-driven engineering in practice. In this respect, on the one hand, dedicated coevolution languages have been proposed for migrating models conforming to an initial metamodel to models conforming to a revised metamodel with the drawback of requiring to learn a new language. O...

Purpose The paper aims to conceptualize how behavioral shifts in times of crisis drive the transformation value co-creation. Design/methodology/approach Referencing two empirical contexts, explores digital service platforms facilitate changes actors’ mental models and institutional arrangements (legal, social, technological) that co-creation ecosystems. Findings proposed conceptual framework co...