Maximizing the conversion of biogenic carbon feedstocks into chemicals and fuels is essential for fermentation processes as feedstock costs and processing is commonly the greatest operating expense. Unfortunately, for most fermentations, over one-third of sugar carbon is lost to CO2 due to the decarboxylation of pyruvate to acetyl-CoA and limitations in the reducing power of the bio-feedstock. ...

Clostridium ljungdahlii ( C. , CLJU) is natively endowed producing acetic acid, 2,3-butandiol, and ethanol consuming gas mixtures of CO 2 CO, H (syngas). Here, we present the syngas-based isobutanol formation using harboring recombinant amplification “Ehrlich” pathway that converts intracellular KIV to isobutanol. Autotrophic production was studied analyzing two different strains in 3-L gassed ...

Syngas fermentation via the Wood-Ljungdahl (WL) pathway is a promising approach for converting gaseous pollutants (CO and CO2) into high-value commodities. Because WL involves several enzymes with trace metal components, it requires an adequate supply of micronutrients in medium targeted bioprocessing such as bioethanol production. Plackett-Burman statistical analysis was performed to examine m...

The current energy model based on fossil fuels is coming to an end due to the increase in global energy demand. Biofuels such as ethanol and butanol can be produced through the syngas fermentation by acetogenic bacteria. The present work hypothesizes that formate addition would positively impact kinetic parameters for growth and alcohol production in Clostridium ljungdahlii PETC and Clostridium...

The integration of gasification with syngas fermentation is still in an early stage development, where many questions exist concerning the quality required. challenge to define conditions that will provide a H2:CO:CO2 ratio suitable for fermentation, as well identify and remove compounds can inhibit performance microorganisms. right balance between line up minimal gas cleaning needs be assessed...