Background: Clostridium chauvoei is the etiological agent of blackleg as an endogenous infection in cattle.  Flagella have been known to play a critical role in the protective immunity of animals to clostridial infections. C.chauvoei has two copies of fliC gene, namely fliA and fliB. OBJECTIVES: The aim of this study was the determination and nucleotide sequence analysis of both copies of fliC ...

Clostridium chauvoei CH3 and Kad1 strains were found to cause marked changes in the blood parameters during the course of blackleg disease. These changes displayed by CH3 were found to be more marked than the local Kad1 strain. Results of changes in the haematological values in calves infected with blackleg organisms, showed an increase in RBC, PCV, Hb and the total leukocyte count. MCHC and MC...

Improved conventional PCR techniques are required for the rapid on-site detection of human and animal diseases. In this context, a PCR method using dry-stored reagents intended for the detection of Clostridium spp. is presented. Basic PCR reagents (BSA, PCR buffer, MgCl₂ and primers), which were dried on polyolefin matrices, showed stability at ambient temperatures for up to 10 months without a...