Droplet digital polymerase chain reaction (ddPCR) is a novel molecular biology technique providing absolute quantification of target nucleic acids without the need for an external calibrator. Despite its emerging applications in medical diagnosis, there are few reports of its use for the detection of plant pathogens. This work was designed to assess the diagnosis potential of the ddPCR for abso...

This study describes the use of electroporation for transforming Xanthomonas axonopodis pv. citri (Xac), the causal agent of citrus (Citrus spp.) canker. It also evaluates the methodology used for this species under different electrical parameters. The bacterium used in the study (Xac 306) was the same strain used for recent complete sequencing of the organism. The use of a plasmid (pUFR047, ge...

Transcriptional activator-like (TAL) effectors of plant pathogenic bacteria function as transcription factors in plant cells. However, how TAL effectors control transcription in the host is presently unknown. Previously, we showed that TAL effectors of the citrus canker pathogen Xanthomonas citri, named PthAs, targeted the citrus protein complex comprising the thioredoxin CsTdx, ubiquitin-conju...

Citrus canker (caused by Xanthomonas citri subsp. citri (ex Hasse) Gabriel et al.) is a disease that affects several citrus species and causes erumpent, necrotic lesions on leaves, shoots, branches, and fruit. Yield loss due to fruit and leaf drop can be substantial, and on fruit the unsightly blemishes leads to reduced marketability (14,15,34). The disease is widespread in citrus growing regio...

IntRODUctIOn Compared with the rest of the world, the California citrus industry is relatively free of diseases that can impact growers’ profits. Unfortunately, exotic plant pathogens may become well established before they are recognized as such. This is primarily because some of the initial symptoms mimic other diseases, mineral deficiencies, or toxicities. In addition, development of disease...

Citrus exports to foreign markets are severely limited today by fruit diseases. Some of them, like citrus canker, black spot and scab, are quarantine for the markets. For this reason, it is important to perform strict controls before fruits are exported to avoid the inclusion of citrus affected by them. Nowadays, technical decisions are based on visual diagnosis of human experts, highly depende...

In this study, visible-near infrared spectroscopy and mid-infrared spectroscopy were compared to evaluate their applicability in classifying citrus leaves infected with canker and Huanglongbing (HLB) from healthy citrus leaves. The visible-near infrared spectra in the range 350-2,500 nm and mid-infrared spectra in the range of 5.15-10.72 μm were collected from healthy and diseased (canker, HLB)...

Xanthomonas citri subsp. citri causes citrus canker. The Asiatic strain has a broad host range, whereas the Wellington variant has a restricted host range. Here, we present the complete genome of X. citri subsp. citri strain A(W)12879. This study lays the foundation to further characterize the mechanisms for virulence and host range of X. citri.

abstract citrus bacterial canker disease caused by xanthomonas axonopodis pv. citri is one of the most important diseases of citrus in south of iran. in this research, the samples of bacterial flora of citrus leaves were collected from various citrus growing areas of hormozgan, jiroft and mazandaran.the epiphytic bacteria were isolated from those leaves during 2000 and 2001 years. from 453 isol...

Herbaria are important resources for the study of the origins and dispersal of plant pathogens, particularly bacterial plant pathogens that incite local lesions in which large numbers of pathogen genomes are concentrated. Xanthomonas axonopodis pv. citri (Xac), the causal agent of citrus bacterial canker disease, is a notable example of such a pathogen. The appearance of novel strains of the pa...