Photo catalytic TiO2 coatings with well defined honeycomb structure were successfully self-assembled on glass substrates by a simple “dip and burn sol-gel method”. The crux of this new method is that the starting material for the sol-gel coating (the water solution of ammonium titanium citrate) was “doped” with “tea-leaf extracts”. Tea-leaf extracts were completely dissolved in the water soluti...

Trans-aconitate synthesis via citrate dehydrase was determined in crude extracts of maize (Zea mays L.) coleoptiles. Two molecular forms of this enzyme were purified by substrate-specific elution from DEAE-cellulose, ammonium sulfate precipitation, and gel filtration. Each molecular form migrates as a single band in isoelectric focusing. Gel filtration and sodium dodecyl sulfate electrophoresis...

The tricarboxylate transporter has been purified in reconstitutively active form from rat liver mitochondria. The transporter was extracted from mitoplasts with Triton X-114 in the presence of cardiolipin and citrate and was then purified by sequential chromatography on hydroxylapatite, Matrex Gel Orange A, Matrex Gel Blue B, and Affi-Gel 501. Analysis of the purified material via sodium dodecy...

radionuclide is associated with transferrin, apart from a weak binding to 0-lipoprotein. The thermodynamic con stant for gallium was estimated and indicated that gallium citrate in the presence of serum proteins occurred in a colloid form bound to transferrin. Ultracentrifugation of tumor homogenates obtained from patients who had received i.v. injections of 67Ga citrate for scintigraphy demons...

The citrate utilization determinant from transposon Tn3411 has been cloned and sequenced, and its polypeptide products have been characterized in minicell experiments. The nucleotide sequence was determined for a 2,047-base-pair BglII restriction endonuclease fragment that includes the citrate determinant. This region contains an open reading frame that would encode a 431-amino-acid very hydrop...

Acetyl-CoA carboxylase, a major rate-limiting enzyme for fatty acid synthesis, is subject to acute regulation by both allosteric modulation and covalent enzyme phosphorylation. Because citrate activation of the enzyme in vitro requires citrate concentrations far in excess of intracellular levels, we have attempted to identify other ligands which might mediate carboxylase activity. Heated liver ...

Abstract The reliable sampling of root exudates in soil-grown plants is experimentally challenging. This study aimed at developing a citrate and mapping technique with millimetre-resolution using DGT (diffusive gradients thin films) ZrOH-binding gels. Citrate adsorption kinetics, capacity, stability ZrOH gels were evaluated. applied to generate 2D maps exuded by white lupin roots grown rhizotro...