Identification and Characterization of a New Metallo-Lactamase, IND-5, from a Clinical Isolate of Chryseobacterium indologenes Mariagrazia Perilli, Bibiana Caporale, Giuseppe Celenza, Cristina Pellegrini, Jean Denis Docquier, Marilina Mezzatesta, Gian Maria Rossolini, Stefania Stefani, and Gianfranco Amicosante* Dipartimento di Scienze e Tecnologie Biomediche, Università di L’Aquila, I-67100 L’...

l objective: To test the antimicrobial effectiveness of a silver alginate dressing on opportunistic pathogens, namely meticillin-sensitive Staphylococcus aureus (mSSa) and meticillin-resistant Staphylococcus aureus (mrSa), Klebsiella spp., Enterococcus faecalis, Enterococcus faecium, Pseudomonas aeruginosa, Escherichia coli, Enterobacter sakazakii, Enterobacter cloacae, Serratia marcescens, Chr...

Background: Bacterial Extracellular Polymeric Substances (EPS) are environmental friendly and versatile polymeric materials that are used in a wide range of industries such as: food, textile, cosmetics, and pharmaceuticals. To make the production process of the EPS cost-effective, improvements in the production yield is required which could be implemented through application of processes such a...

A new natural IND-type metallo-beta-lactamase variant, IND-5, was identified in a clinical isolate of Chryseobacterium indologenes. IND-5 shared 92.8% and 92.4% amino acid homology with IND-1 and IND-3, respectively. Purified enzyme (pI = 8.8, M(r) = 25,000) was able to hydrolyze penicillins, some narrow- and expanded-spectrum cephalosporins, and carbapenems but not monobactams.

Osburn, Brown and Werkman (1937) observed a critical pH level in the dissimilation of glucose by Clostridium butylicum near 6.3. Above this level butyl alcohol and isopropyl alcohol were not formed. The significance of this critical pH was attributed to the fact that in mixtures of acetic (or butyric) acid and its sodium salt below 6.3 there will be "free" acid, i.e., acid which can be distille...