Separation of ten sedative- hypnotic drugs was performed by RP-HPLC using mixed micellar mobile phase. Effect of temperature, type and amount of organic modifier in mobile phase on efficiency (N) and asymmetry factor (B/A) showed that, the appropriate conditions for a good separation were 35°C and 7% (V/V) butanol in mobile phase.  Variations of selectivity factor versus butanol concent...

Today, 2D online or offline liquid chromatography/mass spectrometry is state of the art for the identification of proteins from complex proteome samples in many laboratories. Both 2D liquid chromatography methods use two orthogonal liquid chromatography separation techniques. The most commonly used techniques are strong cation exchange chromatography for the first dimension and reversed phase s...

First part of review Micellar liquid chromatography is devoted to fundamentals and problems of retention modeling and optimization of separation. The 117 literature references have been critically analyzed. The known conceptual, mechanistic and empirical retention models are reviewed, classified and discussed. The methodologies of optimization of separation in high-performance liquid chromatogr...

The requirements of liquid chromatography (LC) separation systems to separate proteins are often different from those of small molecules as the inherent properties of proteins can result in loss by adsorption, precipitation at zero charge or broad and asymmetric peaks in liquid chromatography. This article looks at the specific separation problems of proteins; the advantages and disadvantages o...

Since the turn of the twentieth century, it has been recognized that many drugs, agrochemicals, food additives and fragrances are chiral compounds and their chirality may considerably affect their bioactive behavior [1, 2]. The demand has been increasing for new analytical methods with high selectivity and efficiency. Chiral separation has become an attractive and important field in the separat...

the aim of this research is to develop a silica gel and alumina column chromatography for separation of polycyclic aromatic hydrocarbons (pahs) and polychlorinated biphenyls (pcbs) into two groups. this is achieved by optimizing the cut-off volume of eluting solvent and weight ratio of silica gel and alumina. in this process mixture of pahs and pcbs divide in two groups and simultaneously other...

In this study, a new silica gel based adsorbent was fabricated and its ability in separation and quantification of alkanes mixture was investigated. Silica gel (SiO2) is a polar absorbent which is mainly used to separate polar compounds. Also, the carbon materials such as activated carbon and recently carbon nanotube (CNTs), have been widely used for separation of nonpolar materials. Carbon nan...

The aim of this research is to develop a silica gel and alumina column chromatography for separation of polycyclic aromatic hydrocarbons (PAHs) and polychlorinated biphenyls (PCBs) into two groups. This is achieved by optimizing the cut-off volume of eluting solvent and weight ratio of silica gel and alumina. In this process mixture of PAHs and PCBs divide in two groups and simultaneously o...