A histochemical staining technique for cholinesterase is reported for the study of corneal nerves in whole-thickness rabbit cornea. The technique is relatively simple, yields reproducible results, allows good definition of the nerve fiber pattern, and provides the investigator, using a binocular microscope, with the advantage of three-dimensional study. When tissue is stained for specific choli...

Fifteen patients demonstrating unexpected prolonged apnoea lasting several hours after succinylcholine have been treated by a new preparation of human serum cholinesterase. Adequate spontaneous respiration was re-established in an average period of ten minutes after the injection. In 12 patients biochemical genetic examinations confirmed the presence of an atypical serum cholinesterase. In thre...

Conflicting data have appeared in the literature regarding serum cholinesterase and blood pressure. The studies reported here were undertaken to establish or refute the existence of a direct relationship between these two factors. Two types of studies were performed. First, serum cholinesterase activity was measured in 63 unselected hypertensive patients. Second, a selected group of seven hyper...

The cerebrospinal fluid (CSF) of normal humans contains both true and pseudocholinesterases and choline acetyl transferase (choline acetylase) detectable by radiochemical assay. Elevations of total cholinesterase, pseudocholinesterase, and true cholinesterase occur in brain tumors, meningitis, Guillain-Barre disease, hydrocephalus, and brain abscess. However, the non-specificity and inconstancy...

Purified serum cholinesterase 90 mg was administered to a patient with cholinesterase deficiency. The effect on the action of suxamethonium was studied. The half-life (44.7 h), time constant (64.5 h), rate constant (0.0155 h-1) and distribution volume (2740 ml) of the cholinesterase activity were determined.

BACKGROUND Alzheimer's disease (AD) is characterized by a progressive memory loss that leads to a profound emotional disturbance in later stages. As no safe and effective drug is yet available for the treatment of AD, secondary metabolites from plants may be instrumental in meeting this challenge. Keeping in view this point we evaluated sesquiterpenes of medicinal plant Amberboa ramosa for thei...

BACKGROUND Plasma cholinesterase activity is known to be correlated with plasma triglycerides, HDL- and LDL-cholesterol, and other features of the metabolic syndrome. A role in triglyceride metabolism has been proposed. Genetic variants that decrease activity have been studied extensively, but the factors contributing to overall variation in the population are poorly understood. We studied plas...

Human cholinesterase exists in two forms--acetylcholinesterase located in tissue microsomes and red blood cells and serum cholinesterase found in serum or plasma. The two enzymes display marked differences in structure, substrate specificity, biological function, and origin. Contemporary methods employ acylthiocholine as substrate for serum cholinesterase and a second coupled reaction of thioch...

The effect of some selected food preservatives on serum cholinesterase was determined. It was found that potassium metabisulphite, methyl parabene and propyl parabene caused a significant increase while sacharine, benzoic acid, salicylic acid, copper carbonate and sorbic acid caused a significant decrease in cholinesterase activity. The behavior of serum cholinesterase activity in response to a...

organophosphate (ops) compounds are widely used in intensive agriculture to improve production, protect crops and control diseases vectors. the main mechanism of toxicity of ops is the inhibition of the cholinesterase enzymes. in this study the acetylcholinestrase (ache), butyrylcholinesterase (bche) and specific acetylcholinestrase (sache) activities as potential biomarkers of exposure to ops ...