A new multiple drug-resistant Chinese hamster ovary cell line, CHO-SMR5, has been isolated which demonstrates a direct correlation between reduced cellular topoisomerase II activity (5-fold reduction) and a low level of resistance (3- to 7-fold) to topoisomerase II inhibitors. This cell line, initially selected for resistance to 9-(4,6-O-ethylidene-beta-D-glucopyranosyl)-4'-demethylepipodophyll...

A new multiple drug-resistant Chinese hamster ovary cell line, ( IK )SMRs, has been isolated which demonstrates a direct correlation between reduced cellular topoisomerase II activity (5-fold reduction) and a low level of resistance (3to 7-fold) to topoisomerase II inhibitors. This cell line, initially selected for resistance to 9-(4,6-0-ethylidene-i8-D-glucopyranosyl)-4'-demethylepipodophyllot...

An outbreak of goatpox was attended in district Durg and surrounding regions of Chhattisgarh state. Dried skin scabs were collected from 250 goats of different age groups showing clinical signs suggestive of pox. The prevalence rate was studied based on severity of clinical signs followed by confirmation with agar gel immuno diffusion (AGID) test. Positive scabs were further processed for virus...

Background Chinese hamster ovary (CHO) cells are the most widely used host for large scale production of recombinant therapeutic proteins. A combination of several gene editing approaches applied to Novartis proprietary CHO cell line resulted in a superior cell line with a significant increase of titer and improved product quality. Inter alia we have surprisingly identified a key protease respo...

1-[(4-Amino-2-methylpyrimidin-5-yl)methyl]-3-(2-chloroethyl)-3- nitrosourea hydrochloride (ACNU), a cancer chemotherapeutic bifunctional alkylating agent, causes chloroethylation of DNA and subsequent DNA strand cross-linking through an ethylene bridge. We isolated and characterized two ACNU-sensitive mutants from mutagenized Chinese hamster ovary cells and found them to be new drug-sensitive r...

Abstract Transparency is widespread in nature, ranging from transparent insect wings to ocular tissues that enable you read this text, and marine vertebrates. And yet, cells tissue models biology are usually strongly light scattering optically opaque, precluding deep optical microscopy. Here we describe the directed evolution of cultured mammalian toward increased transparency. We find mutation...

Background:  With one-third of the world’s population infected, tuberculosis (TB) is one of the most common infectious diseases and a major public health problem, especially in developing countries. The efficacy of the BCG vaccine for controlling the disease in adults is poor. The development of an effective TB vaccine is a global objective. An effective tuberculosis vaccine should s...

Microscopic examination of individual mammalian cells embedded in agarose, subjected to electrophoresis, and stained with a fluorescent DNA-binding dye provides a novel way of measuring DNA damage and more importantly, of assessing heterogeneity in DNA damage within a mixed population of cells. With this method, DNA double-strand breaks can be detected in populations of cells exposed to X-ray d...

The three malignant melanoma cell lines used are characterized by having high, intermediate, and low quantities of pigment production. The response of these malignant melanoma cells to X-ray in vitro does not correlate with a model of the classically radioresistant tumor cell that clinical experience might predict. The in vitro melanoma cells were only slightly more resistant to X-rays than the...