نتایج جستجو برای: chicken anemia virus
تعداد نتایج: 483998 فیلتر نتایج به سال:
K.G. Lieu and E.-H. Shim contributed equally to this paper. Correspondence to David A. Jans: [email protected] E.-H. Shim’s present address is Dept. of Urology, University of Alabama, Birmingham, AL 35233. Abbreviations used in this paper: CLSM, confocal laser scanning microscopy; FL, full length; IBB, importin- binding; IBBL, importin-–binding–like; IMP, importin; IP, immunoprecipitation...
The antibody to chicken infectious anemia virus (CIAV) was positive in a specific pathogen-free (SPF) chicken population by ELISA test in our previous inspection, indicating a possible infection with CIAV. In this study, blood samples collected from the SPF chickens were used to isolate CIAV by inoculating into MSB1 cells and PCR amplification. A CIAV strain (SD1403) was isolated and successful...
In the present study, we describe the development of a DNA vaccine against chicken anemia virus. The VP1 and VP2 genes of CAV were amplified and cloned into pBudCE4.1 to construct two DNA vaccines, namely, pBudVP1 and pBudVP2-VP1. In vitro and in vivo studies showed that co-expression of VP1 with VP2 are required to induce significant levels of antibody against CAV. Subsequently, the vaccines w...
A new isolate of chicken anemia virus (CAV) was designated GD-1-12. GD-1-12 was isolated from a 12-day-old commercial broiler in Guangdong province, China, in 2012. The GD-1-12 CAV caused high mortality, severe anemia, thymic atrophy, and subcutaneous hemorrhage in commercial broilers. Here, we report the complete genome sequence of GD-1-12 CAV and comparison with the complete genome sequence o...
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